FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437033
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA26660.610022584106921No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT23580.5395473568357538No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG21940.5020215864705869No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC17750.40614781950104456No Hit
CAATTCGTTGGCACACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTT17580.40225795306075285No Hit
CATACAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGCA16160.3697661275006693No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA16090.36816441778996095No Hit
ATCTAAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG14850.3397912743431274No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12910.29540103378921045No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC11600.26542618063166856No Hit
CCTTATATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACT11560.2645109179398352No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGAGTAAAAGGGGCAGCA11250.25741763207812685No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTCAT10980.2512396089082518No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10150.23224790805270998No Hit
GTGTGTAGCATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG10010.22904448863129329No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG9820.22469699084508493No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9700.2219512027695849No Hit
CTCTATGGCAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC9540.21829015200225155No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8890.20341713325995975No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT8800.20135779220333475No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8800.20135779220333475No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8790.20112897653037642No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAC8180.187171220479918No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8100.1853406950962513No Hit
GCTACACACTGTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGT8040.1839678010585013No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACACC7900.1807643816370846No Hit
GAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAATA7840.1793914875993346No Hit
CTCTTATATAAGATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT7760.17756096221566792No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7540.17252701741058454No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT7540.17252701741058454No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7530.17229820173762622No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC7240.16566254722183452No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT7220.16520491587591785No Hit
CTATTTACACACTCTATGGCAGGCGGGTATCTTATATAAGAGAGAAACAA7200.16474728453000118No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA7150.16360320616520949No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7120.1629167591463345No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7040.1610862337626678No Hit
CCCAAAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAAC7010.1603997867437928No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG6990.15994215539787612No Hit
GCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT6790.15536584193870945No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6660.15239123819025108No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT6630.15170479117137609No Hit
CTGTCTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6620.15147597549841774No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA6610.15124715982545942No Hit
AACGCATTGTGGGTCTTTTGGGTTTTGCTGCCCCTTTTACTCAATGTGGT6600.15101834415250107No Hit
CCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTCATGGGCTATG6490.1485013717499594No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6460.1478149247310844No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA6370.14575558367445937No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT6370.14575558367445937No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6280.14369624261783434No Hit
CTGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAGAGTTGC6230.14255216425304268No Hit
CTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAGAGTTGC6100.1395775605045843No Hit
ATTTTGTGGTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACA6090.139348744831626No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAGTAAT6060.138662297812751No Hit
GGATAACCACATTGAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6060.138662297812751No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5940.13591650973725097No Hit
TCCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5820.13317072166175095No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5810.1329419059887926No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5710.13065374925920925No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG5630.1288232238755426No Hit
GTGTTGTTTCTCTCTTATATAAGATACCCGCCTGCCATAGAGTGTGTAAA5570.12745032983779256No Hit
CTTATATAAGATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT5550.1269926984918759No Hit
CCTTTACCCCGTTGTCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5490.1256198044541259No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5360.12264520070566753No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5360.12264520070566753No Hit
GGGTTACTCTCTGAATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT5330.12195875368679254No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA5320.12172993801383421No Hit
GCGTTCTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGT5310.12150112234087584No Hit
GTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAACATACATTTACA5300.12127230666791752No Hit
GTGTAAATGTATGTTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA5280.12081467532200085No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAAGCCTTGTAAGTTGGCGA5260.12035704397608418No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA5250.12012822830312585No Hit
GAGATACAGTAATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACT5190.11875533426537585No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5160.11806888724650083No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5150.1178400715735425No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTACTGTTGTTAG5090.1164671775357925No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCGAGGGGGAACCACCGTG5060.1157807305169175No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5040.11532309917100082No Hit
GGACTCTCTCGTCCCCTCCTCCGGCTGCCGTTTCGACCGACCACGGGGCG5020.11486546782508414No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4940.11303494244141747No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4900.11211967974958413No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4880.11166204840366745No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4850.11097560138479245No Hit
GTCCCGTGCTGGTGGTTGAGGATCCTGGAAGTAGAGGACAAACGGGCAAC4720.1080009976363341No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA4710.10777218196337576No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4670.10685691927154242No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4670.10685691927154242No Hit
CTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCCGTGGA4600.10525520956083406No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCTTTTCTGTGTAAACAATACC4570.10456876254195907No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4570.10456876254195907No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCGTCCGTGG4540.10388231552308407No Hit
CACTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCA4520.10342468417716741No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGACTCTA4520.10342468417716741No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4510.10319586850420907No Hit
GTATGGATCGGCAGAGGAGCCGTAAAGGTTCCACGCATGCGCTGATGGCC4480.10250942148533407No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGTCCGGCAACGGCCAGGTC4460.10205179013941738No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4430.10136534312054238No Hit
GAGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGGCACACT4420.10113652744758404No Hit
AGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGTCCGGCAA4380.10022126475575072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)