FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620649
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43950.7081297158297202No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA31460.5068887567691239No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT20090.3236934241415035No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT16600.2674619632030343No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14690.2366877252682273No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14110.22734266872257908No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTCTGT13280.21396957056242738No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG12230.19705179578151255No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT12050.19415160581907004No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT11700.1885123475587651No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11580.1865788875838034No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC11340.18271196763388No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT11090.1786839260193765No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT10400.16756653116334674No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10370.16708316616960633No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC9910.15967156959891984No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9730.1567713796364773No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT9710.15644913630731702No Hit
GTACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9700.15628801464273687No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9420.15177660803449294No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9150.14742632309082912No Hit
GTATGGTGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATATAG9070.146137349774188No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT9060.14597622810960784No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC9030.14549286311586743No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8830.1422704298242646No Hit
AATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTTGTCT8660.1395313615264022No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC8540.13759790155144053No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8480.13663117156395968No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT8370.1348588332535781No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA8310.13389210326609724No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8270.1332476166077767No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8270.1332476166077767No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8240.1327642516140363No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG7760.1250304117141895No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7710.1242248033912888No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7520.1211634917642661No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7460.12019676177678527No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG7270.1171354501497626No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7250.1168132068206023No Hit
GTTAACAGGAAGTTTTCTAAAACAATCTTTGATTTTCTGTATGATGTGAT7240.11665208515602217No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7010.11294628687067891No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA6950.11197955688319806No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC6920.11149619188945765No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6790.10940161024991582No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6730.10843488026243497No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6730.10843488026243497No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC6720.10827375859785483No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGATTGT6710.10811263693327469No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAAC6670.10746815027495413No Hit
CTCCGAAAGACACCAAATACTCAATGACCGTTTCTCTTCCAAAAGTGAGA6630.10682366361663355No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG6590.106179176958313No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6500.10472908197709171No Hit
CCTAATAATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACA6460.10408459531877116No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT6390.10295674366671016No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6360.10247337867296975No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA6280.10118440535632862No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA6270.10102328369174848No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6270.10102328369174848No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA6260.10086216202716833No Hit
GTCCCGTACTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6250.10070104036258819No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC6220.10021767536884778No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6210.10005655370426764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)