FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585435
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG31790.5430150230170728No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT20540.3508502224841358No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17860.3050722966682894No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG15650.2673225891858191No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13660.2333307711359929No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13290.22701068436290964No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12930.22086141074585566No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12900.22034897127776784No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12060.2060006661713085No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11550.19728919521381535No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11460.19575187680955186No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG11430.19523943734146404No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT11220.1916523610648492No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA10490.17918300067471196No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG10490.17918300067471196No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10210.1744002323058922No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA9800.16739689290869184No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9650.16483469556825267No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9310.15902704826325723No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9050.15458590620649604No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8910.15219452202208614No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8830.1508280167738519No Hit
GTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT8780.14997395099370553No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8740.14929069836958844No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8720.14894907205752986No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA8360.14279979844047588No Hit
GTCTCCACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT8350.14262898528444662No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7960.1359672721993048No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7960.1359672721993048No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7820.1335758880148949No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7710.13169694329857284No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7490.12793905386592877No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7420.1267433617737238No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7390.12623092230563598No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7180.12264384602902116No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7120.12161896709284549No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACCGAGT7120.12161896709284549No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6940.1185443302843185No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6890.11769026450417211No Hit
ACCGCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT6770.11564050663182078No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6760.11546969347579152No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT6740.11512806716373294No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6730.11495725400770367No Hit
GATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC6690.11427400138358655No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6680.1141031882275573No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6600.11273668297932307No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6590.1125658698232938No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6540.1117118040431474No Hit
TCGCCGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT6440.11000367248285463No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6370.10880798039064969No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6330.10812472776653259No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6300.10761228829844474No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6270.1070998488303569No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA6260.10692903567432763No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC6230.1064165962062398No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6200.10590415673815197No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6090.10402521202182993No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6000.10248789361756643No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5980.10214626730550788No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5940.10146301468139077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)