FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472198
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19560.4142330124227549No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17120.3625597736542721No Hit
GTGTAAAAGGTGCAGCAAAGCCTAAAAGACCCACAATTCTTTGACATACT15390.32592260026514297No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA13730.2907678558570769No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13730.2907678558570769No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12780.27064917682836437No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTAAGTACAAT12530.26535478761028214No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA11290.23909461708859417No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA11030.23358845230178868No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10000.2117755687232898No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA9790.2073282817801007No Hit
GCCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9780.20711650621137745No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9650.20436342381797468No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT9550.20224566813074177No Hit
GTACACAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA9290.19673950334393622No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9060.19186866526330054No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9000.1905980118509608No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTAAGTACAATA8820.18678605161394163No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA8720.18466829592670872No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT8450.1789503555711799No Hit
TGATTAACTGCATGTTCAGGATAATAAGGTTTAATACCTTTATCCAAGGG8360.1770443754526703No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8300.17577372204033054No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAA8290.17556194647160725No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7910.16751447486012225No Hit
CAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAA7900.16730269929139896No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCGATGTCAACGACCGACCT7640.16179653450459341No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATGAACTGCATGTTCAGG7530.15946700324863722No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7510.15904345211119064No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATAC7430.15734924756140434No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA7200.15247840948076866No Hit
GCTTTGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6860.1452780401441768No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTATTCG6790.14379561116311376No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6680.1414660799071576No Hit
CATGTATACAATCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6630.14040720206354115No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAA6610.13998365092609458No Hit
GGAACATATTGTACTTAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAA6410.13574813955162876No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT6360.13468926170801232No Hit
GTCTATTAACAGGCAATTTTCGAAAACATTGCTTGATTTTAAGTACAATA6320.13384215943311914No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC6320.13384215943311914No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTTAAA6300.13341860829567256No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6220.13172440374588626No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6190.1310890770397164No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6100.1291830969212068No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6060.12833599464631362No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6020.12748889237142047No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6020.12748889237142047No Hit
GTATACATTTGAACCCCAATAAAACCAAACGTTGGGGATACTCCCTTAAC5940.12579468782163414No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5940.12579468782163414No Hit
CTTTTAGGCTTTGCTGCACCTTTTACACAATGTGGCTATCCTGCCTTGAT5750.12177095201589164No Hit
GGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT5730.12134740087844506No Hit
TAGCCACATTGTGTAAAAGGTGCAGCAAAGCCTAAAAGACCCACAATTCT5700.1207120741722752No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5600.1185943184850423No Hit
GTCTTTGGGTATACATTTGAACCCCAATAAAACCAAACGTTGGGGATACT5540.11732366507270255No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC5490.1162647872290861No Hit
GGATAGCCACATTGTGTAAAAGGTGCAGCAAAGCCTAAAAGACCCACAAT5410.11457058267929979No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5280.11181750028589701No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5170.10948796902994083No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCT5150.10906441789249426No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5080.10758198891143121No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG5060.10715843777398464No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5060.10715843777398464No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5060.10715843777398464No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5050.10694666220526135No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5020.10631133549909148No Hit
GATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA5010.1060995599303682No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4990.1056760087929216No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC4960.10504068208675174No Hit
GCGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGAC4960.10504068208675174No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTAG4910.1039818042431353No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4870.10313470196824215No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4850.10271115083079557No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4850.10271115083079557No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTAG4840.10249937526207226No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGT4840.10249937526207226No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATACCTTTATCCAAGGGC4820.10207582412462568No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTAAGT4820.10207582412462568No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4770.10101694628100924No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4770.10101694628100924No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG4770.10101694628100924No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4750.10059339514356265No Hit
GGGTATACATTTGAACCCCAATAAAACCAAACGTTGGGGATACTCCCTTA4730.10016984400611607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)