FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472198
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19490.41275058344169185No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16590.3513356685119378No Hit
GTGTAAAAGGTGCAGCAAAGCCTAAAAGACCCACAATTCTTTGACATACT15330.32465194685280324No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14170.30008598088090166No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13550.2869558956200577No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12500.26471946090411225No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTAAGTACAAT12430.26323703192304926No Hit
GCCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT10150.21495220225413916No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10130.2145286511166926No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA9950.21071669087967337No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGATTTTAAGTACAATA9610.20351632154308152No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA9520.20161034142457188No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA9270.19631595220648965No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9260.1961041766377664No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8920.1889038073011745No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8880.18805670502628136No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT8740.1850918470641553No Hit
TGATTAACTGCATGTTCAGGATAATAAGGTTTAATACCTTTATCCAAGGG8690.18403296922053886No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8510.18022100898351961No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCGATGTCAACGACCGACCT8390.17767970215884016No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAA8240.1745030686279908No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT8240.1745030686279908No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA7920.16772625042884554No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7810.16539671917288934No Hit
GTACACAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7690.16285541234820985No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATGAACTGCATGTTCAGG7330.15523149187417143No Hit
CAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCATGGGATATGTAA7290.15438438959927825No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7280.15417261403055496No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATAC7280.15417261403055496No Hit
GTCTATTAACAGGCAATTTTCGAAAACATTGCTTGATTTTAAGTACAATA7190.15226663391204537No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7100.15036065379353578No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT7050.1493017759499193No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCT7030.14887822481247273No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTATTCG6720.14231318218205075No Hit
GCTTTGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6540.13850122194503153No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC6530.13828944637630824No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6410.13574813955162876No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAA6370.1349010372767356No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6340.13426571057056574No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6270.1327832815895027No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTTAAA6270.1327832815895027No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA6260.1325715060207794No Hit
CATGTATACAATCAAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6210.13151262817716297No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6210.13151262817716297No Hit
CTTTTAGGCTTTGCTGCACCTTTTACACAATGTGGCTATCCTGCCTTGAT6020.12748889237142047No Hit
GGAACATATTGTACTTAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAA5880.12452403440929441No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTAG5600.1185943184850423No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5520.11690011393525597No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5480.11605301166036282No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG5470.11584123609163953No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5450.11541768495419294No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5450.11541768495419294No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5420.11478235824802308No Hit
GTCTTTGGGTATACATTTGAACCCCAATAAAACCAAACGTTGGGGATACT5400.1143588071105765No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5370.11372348040440663No Hit
GTATACATTTGAACCCCAATAAAACCAAACGTTGGGGATACTCCCTTAAC5350.11329992926696006No Hit
ATATTGTACTTAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA5310.11245282699206688No Hit
GGATAATAAGGTTTAATACCTTTATCCAAGGGCAAATATTTGGTAAGGTT5300.11224105142334359No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5270.11160572471717373No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5270.11160572471717373No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTAG5240.11097039801100386No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC5230.11075862244228057No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG5200.1101232957361107No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATACCTTTATCCAAGGGC5140.10885264232377097No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5100.10800554004887782No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5000.1058877843616449No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4960.10504068208675174No Hit
GGATAGCCACATTGTGTAAAAGGTGCAGCAAAGCCTAAAAGACCCACAAT4960.10504068208675174No Hit
CTATCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCT4930.10440535538058188No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4930.10440535538058188No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4910.1039818042431353No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACT4880.10334647753696544No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4880.10334647753696544No Hit
GGGTATACATTTGAACCCCAATAAAACCAAACGTTGGGGATACTCCCTTA4870.10313470196824215No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT4840.10249937526207226No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4770.10101694628100924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)