FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659212
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN69071.0477661207623647No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA64430.9773790525657907No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT20520.3112807412486423No Hit
TCTTTACACTGCTCCTATAAATTGAATTATTATGTACTCTTTTGAATCTA19190.29110513764919327No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC17190.26076588411618723No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT16490.25014714537963506No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT15920.24150045812272836No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15160.22997154178018603No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC14400.21844262543764373No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14280.21662227022566338No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT13860.2102510269837321No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC13840.209947634448402No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG13780.20903745684241185No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG13490.20463826508012595No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT13290.20160433972682537No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCAT13000.19720514796453947No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT12010.18218721746570146No Hit
GTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCTAATATACATTTACA11930.18097364732438123No Hit
CATTTACATCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT11040.16747267950219352No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT10740.16292179147224262No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA10190.15457849675066596No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9940.1507860900590402No Hit
CATCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT9840.1492691273823899No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTC9180.13925717371649787No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA9070.13758851477218256No Hit
CTTTAACCCTCATTGGAAAACACCCTTTTTTCCTAATATACATTTACATC8490.1287901312476108No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCTAAT8460.1283350424446157No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC8400.1274248648386255No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATTTACACACTCTATGGA8120.12317736934400465No Hit
GCTTGGGACTGTCTCGTCCCCTTCTCCGTCTGCCGTTTCGTCCGACCACG8120.12317736934400465No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA8110.12302567307633963No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT7990.12120531786435926No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7970.12090192532902921No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT7920.12014344399070406No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC7800.11832308877872369No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGATAATAAGGTTTAATAC7730.11726121490506847No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7490.11362050448110775No Hit
GCCTAGCAGCCATGGAAACGATGTGTATTTGCGGGAGAGAACAACAGAGT7470.11331711194577769No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7040.10679417243618138No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG7030.10664247616851635No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC7030.10664247616851635No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTTTTTTCCTAATATACA7020.10649077990085132No Hit
ATTATGTACTCTTTTGAATCTAGCTTCTTTCTCTCAGCTTAATTTTTTGA7000.10618738736552125No Hit
GTCCCAAGCGGCCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCGACGGG6970.10573229856252617No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC6750.1023949806738955No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC6730.10209158813856545No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6720.10193989187090041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)