FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511699
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN78051.5253107784068367No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA21260.41547863099204807No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT16500.3224551933851737No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT15770.3081889939202539No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11900.2325585940171859No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT11480.22435064364010873No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT11190.2186832493321269No Hit
CATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT10410.20343991291755506No Hit
GTGTATGATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAGC10190.19914051033908606No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC9890.1932776886411738No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTGTGT9630.1881965765029832No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8920.17432123181792422No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8850.17295324008841134No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8760.17119439357903768No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8740.17080353879917687No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8500.16611328144084705No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT8120.15868704062349154No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8000.15634191194432664No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT7950.1553647749946746No Hit
CTTTCCAATCAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTG7900.15438763804502256No Hit
CATCATACACAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAATAGGC7150.13973058380024195No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT7140.13953515641031153No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7060.13797173729086826No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGTGA7040.13758088251100745No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA6840.13367233471239928No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6810.13308605254260805No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6740.1317180608130952No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6450.12605066650511335No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT6360.12429181999573967No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG6340.12390096521587887No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6320.12351011043601806No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT6220.12155583653671395No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT6190.12096955436692275No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6130.11979699002734029No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6070.11862442568775784No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTACT5880.11491130527908008No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACACAAA5860.11452045049921927No Hit
TTCTATCACTGTTTCCCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA5810.11354331354956723No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA5740.11217532182005437No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT5690.11119818487040233No Hit
GAGTAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5690.11119818487040233No Hit
GGACCTTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGT5670.11080733009054151No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5590.10924391097109824No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5580.10904848358116784No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5520.10787591924158538No Hit
GGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTGTGTATGAT5480.10709420968186376No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5450.10650792751207253No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATTCAGAGAGTAACCCCAT5440.10631250012214212No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC5370.10494450839262925No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT5320.10396737144297721No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT5250.10259937971346436No Hit
GGGTTACTCTCTGAATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT5200.10162224276381232No Hit
AATATACATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG5190.1014268153738819No Hit
CTCCGAAAGACACCAAATATTCTATCACTGTTTCCCTTCCAAAAGTTAGA5180.1012313879839515No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5180.1012313879839515No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5160.10084053320409068No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5140.10044967842422986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)