FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511699
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA19480.3806925555844354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17480.34160707759835374No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT16990.3320311354917637No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTGAATTTTAT15260.29822219703380304No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11770.23001803794809056No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT11710.22884547360850815No Hit
GTGTATGATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACATAGC10900.21301585502414505No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT10850.21203871807449298No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10170.19874965555922525No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10020.19581824471026915No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT9760.19073713257207853No Hit
CAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTGTGT9620.1880011491130528No Hit
CATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT9570.18702401216340075No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT9360.18292003697486217No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9110.17803435222660197No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT8850.17295324008841134No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT8440.16494071710126462No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8300.1622047336422389No Hit
CTTTCCAATCAATAGGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTG8020.15673276672418746No Hit
CATCATACACAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAATAGGC7770.15184708197592725No Hit
CCTCCAGCTTATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGA7570.1479385341773191No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT7270.14207571247940684No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7110.1389488742405203No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTACT7050.13777630990093787No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6960.13601746339156418No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT6780.13249977037281682No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6760.132108915592956No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6680.13054549647351274No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6660.13015464169365193No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6610.1291775047439999No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6560.12820036779434785No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6440.12585523911518295No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6390.12487810216553091No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6340.12390096521587887No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA6180.12077412697699234No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC6180.12077412697699234No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA6170.12057869958706192No Hit
TTCTATCACTGTTTCCCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA6110.11940613524747948No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT6070.11862442568775784No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGAACACATCATACACAAA6040.11803814351796663No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT6040.11803814351796663No Hit
CTCCGAAAGACACCAAATATTCTATCACTGTTTCCCTTCCAAAAGTTAGA6010.1174518613481754No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGTGA6010.1174518613481754No Hit
GGACCTTGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGT6010.1174518613481754No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5780.112957031379776No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG5770.1127616039898456No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA5740.11217532182005437No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5720.11178446704019356No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT5600.10943933836102865No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5520.10787591924158538No Hit
AATATACATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG5470.10689878229193334No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC5450.10650792751207253No Hit
GGCCTATTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTGTGTATGAT5440.10631250012214212No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG5360.10474908100269885No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5340.10435822622283804No Hit
GGGTTACTCTCTGAATTTTATGGGCTATGTCATTGGATGTTATGGGTCAT5320.10396737144297721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)