FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356014
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45491.2777587398248385No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA27590.7749695236704175No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG19920.5595285578657019No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT17430.4895874881324892No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT9020.2533608228889875No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT8380.23538400175274007No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8260.23201334778969368No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT7370.20701433089709956No Hit
ATATTCATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGC7260.203924564764307No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG7140.20055391080126064No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT6970.1957788176869449No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6900.19381260287516783No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA6720.18875662193059822No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC6700.1881948462700905No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6630.18622863145831345No Hit
GGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG6520.1831388653255209No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT6420.18032998702298225No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT6270.17611666956917424No Hit
CTACGAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC6070.17049891296409692No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT6070.17049891296409692No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA5920.1662855955102889No Hit
GGAGACTCTAAGGCATCCCGATACAGAGCTGAGGCGGTGTCTAGAAGAGC5670.15926339975394227No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC5650.15870162409343452No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5640.15842073626318065No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5600.15729718494216519No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT5580.15673540928165747No Hit
TCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAGA5550.15589274579089588No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCACCTCGCAGACGAAGAT5490.15420741880937266No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC5480.15392653097911882No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT5280.14830877437404147No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5250.14746611088327985No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT5230.14690433522277213No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5160.14493812041099507No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5120.1438145690899796No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG5100.14325279342947186No Hit
AATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGACAGGT5090.14297190559921802No Hit
CCTGTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG5030.1412865786176948No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCAGTAATGATTAACT5030.1412865786176948No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5020.14100569078744093No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT4910.13791592465464841No Hit
CTACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTA4890.13735414899414067No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4840.13594970984287133No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4820.1353879341823636No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG4820.1353879341823636No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCTCTAGATGCTGGATCTTC4620.1297701775772863No Hit
ATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAG4610.12948928974703242No Hit
GACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTT4560.12808485059576308No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTGTCCACCA4510.12668041144449374No Hit
CTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC4410.12387153314195509No Hit
CATTTCTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGT4400.12359064531170122No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT4400.12359064531170122No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC4340.12190531833017804No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTACGGGAGAGGACAACAGAGT4330.12162443049992416No Hit
ATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGC4330.12162443049992416No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAA4310.12106265483941643No Hit
GTATATTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACCAGCACG4300.12078176700916256No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4300.12078176700916256No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4230.1188155521973855No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT4200.1179728887066239No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA4190.11769200087637004No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4150.11656844955535456No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4070.11432134691332363No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4020.11291690776205429No Hit
GTTGTTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG4010.11263601993180045No Hit
TTCCTAAAACATTCTTTGATTTTTTGTGTGATGTGTTCCTGTGGCAATGA4000.11235513210154657No Hit
CTATTTACACACTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAA3960.1112315807805311No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3940.11066980512002336No Hit
CTAACAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC3890.10926536596875404No Hit
ACTAACAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGT3860.10842270247799243No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC3860.10842270247799243No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTGCAAG3840.1078609268174847No Hit
AGACTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAA3840.1078609268174847No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3830.10758003898723083No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA3820.10729915115697697No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA3740.10505204851494604No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3690.10364760936367672No Hit
TACTACGAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAG3650.10252405804266124No Hit
ACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACT3640.10224317021240738No Hit
GGCATAATCAATTGCAATCTTCTTTTCTCATTAACTGTGAGTGGGCCTAC3640.10224317021240738No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG3630.1019622823821535No Hit
GAGGAACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACC3590.10083873106113805No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA3590.10083873106113805No Hit
GTCTAGAAGAGCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3570.10027695540063032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)