FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356014
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA26930.7564309268736622No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT18740.5263837938957456No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG18130.5092496362502599No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT10340.2904380164824979No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT9520.2674052144016808No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7740.2174071806164926No Hit
GTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTATA7720.21684540495598487No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT7270.20420545259456088No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG7050.1980259203289758No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT7050.1980259203289758No Hit
ATATTCATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGC6720.18875662193059822No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT6430.18061087485323613No Hit
AGTATATGGTGACCCACAAAATGAGGCGCTATGTGTTGTTTCTCTCTTAT6330.17780199655069745No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA6100.1713415764548585No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT5990.168251810322066No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5870.1648811563590196No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG5830.16375760503800413No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGAATTTCAT5800.1629149415472425No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC5770.16207227805648092No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC5760.16179139022622707No Hit
CTACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTA5740.16122961456571933No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5710.16038695107495773No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5680.15954428758419614No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC5650.15870162409343452No Hit
CTACGAACTTTGCCAGCAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC5610.15757807277241906No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5600.15729718494216519No Hit
GACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTT5590.1570162971119113No Hit
GGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAG5580.15673540928165747No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC5550.15589274579089588No Hit
TCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAGA5540.155611857960642No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5490.15420741880937266No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT5310.14915143786480306No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5290.14858966220429534No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5230.14690433522277213No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA5110.14353368125972574No Hit
AATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGACAGGT5100.14325279342947186No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA5090.14297190559921802No Hit
CATTTCTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGT4970.1396012516361716No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT4860.1365114855033791No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT4810.13510704635210974No Hit
GTATATTGCCCGTTTGTCCTCTAATTCCAGGATCCTCAACCACCAGCACG4790.13454527069160202No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4750.13342171937058656No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT4690.13173639238906334No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT4660.13089372889830175No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCAGTAATGATTAACT4640.130331953237794No Hit
ATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGTTAATGAGAAAAG4550.12780396276550923No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAA4550.12780396276550923No Hit
CCTGTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG4520.12696129927474764No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCTCTAGATGCTGGATCTTC4500.1263995236142399No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG4470.1255568601234783No Hit
ATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGC4440.1247141966327167No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4430.12443330880246282No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4390.12330975748144736No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4350.12218620616043188No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT4320.12134354266967029No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4250.11937732785789322No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT4250.11937732785789322No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCACCTCGCAGACGAAGAT4180.11741111304611616No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4110.11544489823433911No Hit
TTCCTAAAACATTCTTTGATTTTTTGTGTGATGTGTTCCTGTGGCAATGA4090.11488312257383136No Hit
GGAGACTCTAAGGCATCCCGATACAGAGCTGAGGCGGTGTCTAGAAGAGC4040.11347868342256202No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTACGGGAGAGGACAACAGAGT4030.11319779559230818No Hit
GTTGTTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG3920.11010802945951564No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3860.10842270247799243No Hit
CCATATACTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTGTCCACCA3850.10814181464773857No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3770.10589471200570764No Hit
CCTTTGGATAAAACCTAGCAGGCATAATCAATTGCAATCTTCTTTTCTCA3730.10477116068469217No Hit
ACATCGACCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTT3660.10280494587291511No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC3650.10252405804266124No Hit
ACACTCATCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACT3610.10140050672164577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)