FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623997
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67171.076447482920591No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT38760.6211568324847715No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT37360.598720827183464No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG29530.47323945467686546No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC26780.42916872997786853No Hit
GGATAACCACATTGCGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAAT26400.42307895711037075No Hit
GGGTAAAGGTTTAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA25520.40897632520669175No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT21800.3493606539775031No Hit
GGTTTAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG19100.3060912151821243No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC18740.3003219566760738No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC18690.29952067077245564No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT16930.2713154069650976No Hit
GCGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16390.2626615192060218No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT16140.2586550896879312No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA15930.25528968889273507No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT15820.2535268599047752No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT15520.24871914448306642No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAA15350.2459947724107648No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG14750.2363793415673473No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA14330.22964853997695503No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG14220.22788571098899513No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA14160.2269241679046534No Hit
TTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCTAAAAC14130.2264433963624825No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC14020.22468056737452263No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC14010.224520310193799No Hit
ATCAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCT13930.22323825274801No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG13860.22211645248294462No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG13850.221956195302221No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC13830.22163568094077374No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC13740.22019336631426112No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA13690.219392080410643No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC13480.2160266796154469No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA13380.2144241078082106No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT13320.21346256472386888No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA13260.2125010216395271No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13220.2118599929166326No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG13150.21073819265156726No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA13140.2105779354708436No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGTGC12990.20817407775998922No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC12790.20496893414551676No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG12760.20448816260334587No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC12670.2030458479768332No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA12630.20240481925393872No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT12530.20080224744670247No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT12500.2003214759045316No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA12350.1979176181936772No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG12030.1927893884105212No Hit
CTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG12020.19262913122979758No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT11900.19070604506111408No Hit
CCTTTTACGCAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT11540.18493678655506357No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC11460.18365472910927455No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG11440.1833342147478273No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC11350.18189190012131468No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGA11300.18109061421769657No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA11230.17996881395263117No Hit
CATTAAAGCAGGATAACCACATTGCGTAAAAGGTGCAGCAAAACCCAAAA11000.1762828987959878No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT10970.17580212725381694No Hit
GTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATG10910.17484058416947518No Hit
GACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT10700.17147518337427903No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTT10590.16971235438631918No Hit
TAACCACATTGCGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATACG10120.16218026689230877No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA10040.16089820944651978No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC9790.15689177992842915No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG9710.15560972248264013No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT9630.15432766503685114No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTT9500.152244321687444No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9470.15176355014527312No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC9470.15176355014527312No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA9330.14951994961514237No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA9140.1464750631813935No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT8930.14310966238619738No Hit
ATACGTTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCT8920.14294940520547375No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGT8850.14182760494040836No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT8850.14182760494040836No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC8790.14086606185606662No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC8730.13990451877172486No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT8730.13990451877172486No Hit
GGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGAT8710.1395840044102776No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGTCTGT8690.13926349004883037No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA8680.13910323286810675No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTTAGGTATTGTTTACAC8590.1376609182415941No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG8540.13685963233797596No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC8520.13653911797652873No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT8430.13509680335001611No Hit
GTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAG8370.13413526026567435No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG8340.1336544887235035No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA8250.13221217409699085No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG8110.1299685735668601No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA8110.1299685735668601No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC7810.12516085814515135No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA7810.12516085814515135No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7810.12516085814515135No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7760.12435957224153321No Hit
CCTGTTAACAGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT7700.12339802915719146No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT7630.1222762288921261No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7610.12195571453067883No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA7550.12099417144633708No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA7540.12083391426561346No Hit
GTTAACAGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTT7490.12003262836199532No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT7470.11971211400054807No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA7350.11778902783186457No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGAC7300.11698774192824646No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTTAGGT7290.11682748474752282No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7270.11650697038607559No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG7230.11586594166318107No Hit
ACCTAAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG7220.11570568448245745No Hit
GTGTAAACAATACCTAAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7040.1128210552294322No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC7010.11234028368726132No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA6990.11201976932581406No Hit
AATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA6920.1108979690607487No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC6890.11041719751857781No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGG6880.11025694033785419No Hit
ACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCGAGGATT6860.10993642597640695No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6780.10865436853061794No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT6780.10865436853061794No Hit
TCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTT6710.10753256826555256No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6690.1072120539041053No Hit
GCCCATGACCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6640.10641076800048718No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6530.10464793901252731No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6510.10432742465108005No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT6480.10384665310890918No Hit
CCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATC6420.10288511002456743No Hit
AGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAATTT6400.10256459566312018No Hit
CCCACAATACGTTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAG6350.10176330975950204No Hit
TTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACA6320.10128253821733116No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT6310.10112228103660754No Hit
TTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCT6290.10080176667516029No Hit
ACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6280.10064150949443668No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)