FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623997
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66771.070037195691646No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT37740.6048106000509618No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT37630.603047771063002No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG30340.4862202863154791No Hit
GGATAACCACATTGCGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAAT30250.48477797168896647No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC26570.4258033291826724No Hit
GGGTAAAGGTTTAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA25830.4139442978091241No Hit
CAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT23300.3733992310860469No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATGTGAT19410.31105918778455666No Hit
GGTTTAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG19400.31089893060383306No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC17900.2868603534952892No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT17220.2759628652060827No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA17060.2733987503145047No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC16600.2660269200012179No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT15590.24984094474813182No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA15250.24439220060352854No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA15070.2415075713505033No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC14800.2371806274709654No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAA14770.23669985592879453No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC14640.2346165125793874No Hit
GCGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14170.22708442508537702No Hit
GCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG14100.22596262482031162No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC14070.22548185327814074No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA13910.22291773838656273No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG13680.21923182322991938No Hit
AGATCACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTA13630.21843053732630127No Hit
ATCAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCT13600.2179497657841304No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG13570.2174689942419595No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG13520.21666770833834137No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC13510.21650745115761777No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC13460.2157061652539996No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC13380.2144241078082106No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT13260.2125010216395271No Hit
CTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG13240.21218050727807988No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT13120.21025742110939638No Hit
TTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCTAAAAC13000.20833433494071288No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT12970.207853563398542No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAC12710.2036868766997277No Hit
GTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGATG12650.20272533361538597No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA12530.20080224744670247No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC12320.19743684665150635No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG12240.19615478920571736No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA12160.19487273175992834No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG12080.19359067431413934No Hit
GTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCC11950.19150733096473221No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGTGC11810.18926373043460146No Hit
GACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCA11790.1889432160731542No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT11700.18750090144664155No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11610.18605858682012894No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC11220.17980855677190755No Hit
ATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCCATGAA11210.17964829959118395No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG10900.17468032698875155No Hit
GGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACA10770.17259698363934442No Hit
ACATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGA10730.1719559549164499No Hit
CCTTTTACGCAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT10730.1719559549164499No Hit
ACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT10670.17099441183210815No Hit
GACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT10360.16602643922967578No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG10340.16570592486822852No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA10090.16169949535013792No Hit
CCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9750.15625075120553464No Hit
ACAATACGTTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTT9700.15544946530191653No Hit
CATTAAAGCAGGATAACCACATTGCGTAAAAGGTGCAGCAAAACCCAAAA9670.15496869375974565No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA9630.15432766503685114No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT9490.1520840645067204No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT9460.15160329296454952No Hit
GGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTATGAT9250.14823789216935337No Hit
GTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACAT9190.1472763490850116No Hit
CAATACGTTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTT9140.1464750631813935No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT9030.14471223419343363No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC9010.14439171983198637No Hit
GCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACAT8940.143269919566921No Hit
GATTGAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGT8780.140705804675343No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC8760.14038529031389574No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTTAGGTATTGTTTACAC8750.1402250331331721No Hit
ATACGTTGACAGACTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCT8740.14006477595244848No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8710.1395840044102776No Hit
CAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG8700.139423747229554No Hit
TAACCACATTGCGTAAAAGGTGCAGCAAAACCCAAAAGACCCACAATACG8670.13894297568738312No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG8630.1383019469644886No Hit
GTTCAGGAACAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCA8620.13814168978376498No Hit
GTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGT8560.13718014669942324No Hit
ACCCAAAAGACCCACAATACGTTGACAGACTTTCCAATCAATAGGTCTGT8390.1344557746271216No Hit
TTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATGG8370.13413526026567435No Hit
GTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAG8260.13237243127771448No Hit
GTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAAC8220.13173140255482No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC8200.13141088819337274No Hit
GGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGA8030.1286865161210711No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA7980.127885230217453No Hit
CTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA7880.1262826584102167No Hit
GTGAAAGCCTGCTTAGATTGAATACATGCATACAAGGGCATTAAAGCAGG7820.12532111532587495No Hit
GTTAACAGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTT7770.12451982942225683No Hit
GTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGC7620.12211597171140245No Hit
CATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCA7620.12211597171140245No Hit
CCTGTTAACAGACCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT7500.12019288554271895No Hit
ATCTTGGGCTTTCGGAAAATTCCTATGGGAGTGGGCCTCAGCCCGTTTCT7490.12003262836199532No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA7480.11987237118127171No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7440.1192313424583772No Hit
ACCTAAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG7410.11875057091620632No Hit
GCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTA7340.11762877065114095No Hit
TTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC7340.11762877065114095No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGAC7290.11682748474752282No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7270.11650697038607559No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA7260.11634671320535195No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT7250.11618645602462832No Hit
CATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7180.11506465575956296No Hit
GTGTAAACAATACCTAAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7160.1147441413981157No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG7010.11234028368726132No Hit
CTCCCATAGGAATTTTCCGAAAGCCCAAGATGATGGGATGGGAATACAGG7000.11218002650653769No Hit
GTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAAAT6680.10705179672338169No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC6660.10673128236193444No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6650.10657102518121081No Hit
CATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGT6650.10657102518121081No Hit
TTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGTT6510.10432742465108005No Hit
ACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCGAGGATT6460.10352613874746192No Hit
GAACTACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6450.1033658815667383No Hit
GCCCAAGATGATGGGATGGGAATACAGGTGCAATTTCCGTCCGAAGGTTT6430.10304536720529105No Hit
GCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGCACAGACCTGGCC6400.10256459566312018No Hit
CACTTGGCACAGACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTTAGGT6370.1020838241209493No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGATTGAA6250.1001607379522658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)