FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274515
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17360.6323880297980075No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC15970.5817532739558858No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA15260.5558894778063129No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA9560.34825055097171376No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCGCCATGC8370.30490137150975355No Hit
CGGTGGTCGCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7960.28996593993042274No Hit
CATGTATACATGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7810.2845017576453017No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7720.2812232482742291No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA7350.2677449319709306No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAATTTCAT6920.2520809427535836No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6190.22548858896599458No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6170.22476003132797842No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6090.22184580077591387No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6050.2203886854998816No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC5990.21820301258583322No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5750.20946032092963954No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5730.2087317632916234No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC5610.2043604174635266No Hit
ACATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTT5610.2043604174635266No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5490.19998907163542978No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG5440.19816767754038941No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACATGCTAAACAG5280.1923392164362603No Hit
TCCTAATACAAAGACCTTTAACCTGATCTCCTCCCCCAACTCCTCCCACT5260.19161065879824418No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT5240.19088210116022802No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG5190.1890607070651877No Hit
GGTCTATGTCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC5140.18723931297014734No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5100.18578219769411508No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGACAGCACGACAGAAT4850.17667522721891335No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4820.17558239076188917No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4800.174853833123873No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4760.17339671784784072No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4730.17230388139081654No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4700.17121104493379233No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG4670.17011820847676812No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4670.17011820847676812No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA4660.16975392965776004No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT4650.169389650838752No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTGATCTCCTCC4640.1690253720197439No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA4610.16793253556271973No Hit
CCTTTAACCTGATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG4580.1668396991056955No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4480.1631969109156148No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT4400.16028268036355026No Hit
GGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGA4390.15991840154454218No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4360.158825565087518No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT4350.15846128626850992No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGTCAAG4300.15663989217346957No Hit
ACTTATCGGGACTGACAATTCTGTCGTGCTGTCCCGCAAGTATACATCGT4300.15663989217346957No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT4290.1562756133544615No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT4290.1562756133544615No Hit
GCGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT4230.1540899404404131No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4160.1515399887073566No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4140.15081143106934047No Hit
GGATATCCACATTGCGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT4100.14935431579330818No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4090.14899003697430013No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG4060.14789720051727592No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATCA4010.14607580642223558No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGACTA3980.14498296996521137No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT3980.14498296996521137No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3970.14461869114620332No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3960.14425441232719524No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG3960.14425441232719524No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCGTTTTGTTTTG3940.1435258546891791No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3940.1435258546891791No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3900.14206873941314682No Hit
ATGTATACTTGCGGGACAGCACGACAGAATTGTCAGTCCCGATAAGTTTT3890.14170446059413874No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3860.14061162413711456No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG3750.1366045571280258No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3700.13478316303298546No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT3660.13332604775695317No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC3660.13332604775695317No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGA3650.1329617689379451No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3610.13150465366191283No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG3590.1307760960238967No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3580.13041181720488862No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3540.12895470192885633No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3540.12895470192885633No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGTCAAGTG3510.12786186547183215No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTC3490.12713330783381602No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3480.12676902901480794No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3460.1260404713767918No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3430.1249476349197676No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC3430.1249476349197676No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGCGGAACCTTTGTGTC3420.12458335610075952No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG3410.12421907728175147No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA3400.12385479846274339No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGTCAAGTGTTTGCTG3390.12349051964373531No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3380.12312624082472724No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3380.12312624082472724No Hit
GTTTAGCATGTATACATGCATACAAAGGCATTAAAGCAGGATATCCACAT3360.12239768318671111No Hit
ATGTGGTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTATGCC3340.12166912554869497No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATACAGTGGAA3340.12166912554869497No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA3310.12057628909167076No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3310.12057628909167076No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT3270.11911917381563848No Hit
GTATGCATGTATACATGCTAAACAGGCTTTTACTTTCTCGCCAACTTACA3230.1176620585396062No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTGATCT3230.1176620585396062No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA3180.11584066444456588No Hit
ATGTTGTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAG3170.1154763856255578No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3170.1154763856255578No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3120.11365499153051747No Hit
GCTTTAATGCCTTTGTATGCATGTATACATGCTAAACAGGCTTTTACTTT3110.1132907127115094No Hit
CCTAATACAAAGACCTTTAACCTGATCTCCTCCCCCAACTCCTCCCACTC3090.11256215507349325No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3090.11256215507349325No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA3050.11110503979746097No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACATGCTAAAC3020.11001220334043676No Hit
TATCCACATTGCGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG3010.1096479245214287No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA2990.10891936688341257No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA2960.10782653042638836No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC2930.10673369396936416No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG2910.10600513633134802No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT2900.10564085751233994No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC2890.10527657869333189No Hit
ATCTTGGGCTTTCGCAAAATACCTATGGGAGTGGGCCTCAGTCCGTTTCT2890.10527657869333189No Hit
CCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTGC2890.10527657869333189No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG2880.10491229987432381No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG2870.10454802105531574No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTC2860.10418374223630766No Hit
CATGCATACAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGC2850.10381946341729961No Hit
CTCTTATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCGC2850.10381946341729961No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA2810.10236234814126732No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG2810.10236234814126732No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT2800.10199806932225926No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC2770.10090523286523505No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)