FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529748
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA48120.9083564260742845No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC38370.7243066514644698No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG22430.42340886610237316No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA21030.3969812061583999No Hit
GCTTATCGGGACTGACAATTCTGTCGTGCTGTCCCGCAAGTATACATCGT20790.39245075016800435No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC18420.3477124972628495No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACACACT16470.3109025423408866No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG16220.3061833173508914No Hit
ATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC15580.29410210137650356No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA14960.2823984234013153No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT14010.26446536843933344No Hit
CTATTGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAT13670.2580472224529399No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC13440.25370553546214425No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG13170.2486087724729494No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA12820.24200185748695607No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA12630.2384152464945597No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12380.23369602150456445No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11850.2236912645257745No Hit
TGGCCATAGGCCATCAGCGCATGCGCGGAACCTTTGTGTCTCCTCTGCCG11750.22180357452977642No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11580.21859450153657967No Hit
GTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGA11340.21406404554618425No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGACAGCACGACAGAAT10820.2042480575669941No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT10580.19971760157659868No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC10520.19858498757899984No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10180.1921668415926063No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10120.19103422759500743No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC10030.18933530659860914No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT9990.1885802306002099No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT9630.18178454661461677No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9620.18159577761501694No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA9520.17970808761901885No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG9470.1787642426210198No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA9460.17857547362141998No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG9460.17857547362141998No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAG9340.17631024562622227No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCACT9310.17574393862742285No Hit
ATGTATACTTGCGGGACAGCACGACAGAATTGTCAGTCCCGATAAGCTTT9220.17404501763102456No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA9060.1710247136374276No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9010.17008086863942856No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC8920.16838194764303027No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGACGAGAAGGCACAG8850.1670605646458316No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC8830.16668302664663198No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8730.1647953366506339No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG8700.16422902965183447No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8670.16366272265303503No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8550.16139749465783731No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8550.16139749465783731No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG8540.16120872565823752No Hit
GTACAATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCC8410.15875472866343998No Hit
GTCCGGCAGACGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8380.15818842166464053No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT8370.15799965266504074No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATCTTTTGTACAATATGTT8330.15724457666664152No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG8290.15648950066824227No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG8240.15554565567024323No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCACTCAGTA8180.15441304167264436No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGGCA8140.1536579656742451No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8140.1536579656742451No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAATATTTGGTAACATT8080.15252535167664627No Hit
GTCCAAGAGTCCTCTTATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTC8070.15233658267704644No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG7970.15044889268104833No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATCTTTTGT7920.14950504768304929No Hit
GGAACATATTGTACAAAAGATCAAATCATGTTTTAGGAAACTTCCTGTAA7910.1493162786834495No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7840.14799489568625082No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7810.1474285886874514No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7680.14497459169265386No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7660.14459705369345424No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7420.14006659770305882No Hit
GTGCACACGGTCCGGCAGACGAGAAGGCACAGACGGGGAGTCCGCGTAAA7280.1374238317086615No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCACCTCGCAGACGAAGGT7160.13515860371346378No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGACTA7090.1338372207162651No Hit
CCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCA7040.13289337571826604No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG7010.13232706871946662No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6940.13100568572226795No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTTACTTT6930.13081691672266813No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6920.13062814772306833No Hit
CCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA6880.1298730717246691No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT6870.12968430272506928No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG6870.12968430272506928No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG6800.12836291972787062No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6800.12836291972787062No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT6790.1281741507282708No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG6780.127985381728671No Hit
GTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6780.127985381728671No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC6710.12666399873147233No Hit
ATATTGTACAAAAGATCAAATCATGTTTTAGGAAACTTCCTGTAAACAGG6650.12553138473387346No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGACGAGAAGGCACAGACGGGGA6630.12515384673467386No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA6620.12496507773507404No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT6620.12496507773507404No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6610.12477630873547421No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCGTTGT6610.12477630873547421No Hit
GCATAGACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTT6540.12345492573827557No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6520.12307738773907594No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACA6480.1223223117406767No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6440.12156723574227746No Hit
CCCGCAAGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG6440.12156723574227746No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT6430.12137846674267765No Hit
TCCCATCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGG6410.12100092874347804No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6390.1206233907442784No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6320.11930200774707975No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6240.11779185575028125No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG6220.11741431775108165No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA6210.11722554875148183No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTG6180.1166592417526824No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT6180.1166592417526824No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6140.11590416575428318No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6120.11552662775508354No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6020.11363893775908544No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6010.11345016875948565No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT5960.11250632376148659No Hit
CTCTTATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTC5940.11212878576228698No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5930.11194001676268715No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5920.11175124776308737No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC5720.10797586777109115No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5720.10797586777109115No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGG5710.10778709877149134No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA5710.10778709877149134No Hit
ATGTAAGACCGTTGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC5660.1068432537734923No Hit
GCATAAAGGGACTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACATC5580.10533310177669383No Hit
GTTTACGTCCCATCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG5560.1049555637774942No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5530.10438925677869478No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG5520.10420048777909498No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5430.10250156678269667No Hit
AGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTATTT5430.10250156678269667No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGAGATCTACTCGACACC5360.10118018378549802No Hit
GTATACTTGCGGGACAGCACGACAGAATTGTCAGTCCCGATAAGCTTTGC5350.1009914147858982No Hit
CATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGC5340.10080264578629838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)