FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529748
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA33230.6272793856701677No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC32640.6161420146937789No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA21260.40132289314919545No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG19240.3631915552300339No Hit
GCTTATCGGGACTGACAATTCTGTCGTGCTGTCCCGCAAGTATACATCGT18400.3473349592636499No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACACACT15310.2890053383873087No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT13520.25521568745894274No Hit
TGGCCATAGGCCATCAGCGCATGCGCGGAACCTTTGTGTCTCCTCTGCCG13320.25144030746694657No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA12940.24426708548215378No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC12700.23973662949175836No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12370.23350725250496462No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12350.233129714505765No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12120.22878802751496938No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCCATGAA11870.22406880252497413No Hit
CTATTGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAT11450.21614050454178213No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA11260.21255389354938575No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT11260.21255389354938575No Hit
TCCCATCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGG11160.21066620355338767No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG11000.20764589955979068No Hit
GTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC10600.20009513957579833No Hit
GTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG10350.19537591458580308No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10250.19348822458980497No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG10210.19273314859140572No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC10050.18971284459780877No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGACAGCACGACAGAAT9950.18782515460181065No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATA9690.18291716061221563No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA9660.1823508536134162No Hit
ATGTAATTGGGGGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAG9540.18008562561821848No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAG9450.17838670462182019No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC9390.17725409062422132No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9380.17706532162462152No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9360.1766877836254219No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC9330.17612147662662248No Hit
ATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC9210.17385624863142474No Hit
GGAACATATTGTACAAAAGATCAAATCATGTTTTAGGAAACTTCCTGTAA9190.17347871063222514No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG9100.17177978963582685No Hit
ATGTGGATATCCTGCTTTAATGCCTTTGTATGCATGTATACAAGCTAAAC8830.16668302664663198No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG8710.16441779865143424No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8620.16271887765503598No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8560.1615862636574371No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAATATTTGGTAACATT8560.1615862636574371No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8550.16139749465783731No Hit
GTTTACGTCCCATCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG8480.16007611166063865No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATCTTTTGTACAATATGTT8430.15913226666263958No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCT8350.1576221146658411No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG8310.15686703866744187No Hit
GTACAATATGTTCCTGTGGCAAGGAGCCCCAACCCCCAATTACATATCCC8220.15516811767104358No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG8200.15479057967184398No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT8150.15384673467384494No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG7870.14856120268505027No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAAT7850.14818366468585062No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTG7820.1476173576870512No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7730.1459184366906529No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7720.14572966769105308No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGGCA7640.14421951569425462No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATCTTTTGT7610.1436532086954552No Hit
ATATTGTACAAAAGATCAAATCATGTTTTAGGAAACTTCCTGTAAACAGG7580.14308690169665578No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA7580.14308690169665578No Hit
ATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGGCTCTACCGCCCGCTC7530.14214305669865673No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCGGTG7500.1415767496998573No Hit
ATGTATACTTGCGGGACAGCACGACAGAATTGTCAGTCCCGATAAGCTTT7470.14101044270105786No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7420.14006659770305882No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA7400.1396890597038592No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7360.13893398370545995No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG7330.13836767670666053No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG7290.13761260070826128No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACA7190.1357249107122632No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7190.1357249107122632No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7110.1342147587154647No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC7090.1338372207162651No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7090.1338372207162651No Hit
GGCCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGTCTTTTGGGGTTC7080.13364845171666528No Hit
GTCCCGCAAGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACT7020.13251583771906641No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT7010.13232706871946662No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTTACTTT7000.13213829971986682No Hit
GTTGTCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGA6930.13081691672266813No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6890.13006184072426888No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6810.12855168872747041No Hit
CCATCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCGCTTGGGGCT6810.12855168872747041No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6740.12723030573027175No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG6710.12666399873147233No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6600.12458753973587443No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6570.124021232737075No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6570.124021232737075No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCACT6430.12137846674267765No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCACCTCGCAGACGAAGGT6350.11986831474587918No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAATAAATGAAAACAGGAGACTA6350.11986831474587918No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA6320.11930200774707975No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC6310.11911323874747994No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG6290.11873570074828033No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6210.11722554875148183No Hit
CCCGCAAGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG6170.1164704727530826No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGG6160.11628170375348279No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6080.1147715517566843No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC6070.1145827827570845No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6000.11326139975988583No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG5960.11250632376148659No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5940.11212878576228698No Hit
GTCCGGCAGACGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5940.11212878576228698No Hit
CTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTGCACTTCGCTTCAC5940.11212878576228698No Hit
ATGTTGTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5930.11194001676268715No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG5890.11118494076428793No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5880.11099617176468812No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5860.1106186337654885No Hit
CCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGAAGTATGCCTCA5830.11005232676668908No Hit
GTACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5800.10948601976788963No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5720.10797586777109115No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT5720.10797586777109115No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5720.10797586777109115No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5710.10778709877149134No Hit
GTCTGGAGCAAAGCTTATCGGGACTGACAATTCTGTCGTGCTGTCCCGCA5670.10703202277309212No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT5640.10646571577429269No Hit
CCCTTAATTTCATGGGATATGTAATTGGGGGTTGGGGCTCCTTGCCACAG5640.10646571577429269No Hit
GTACAAAAGATCAAATCATGTTTTAGGAAACTTCCTGTAAACAGGCCTAT5580.10533310177669383No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCACTCAGTA5570.10514433277709402No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT5560.1049555637774942No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT5510.10401171877949517No Hit
GTCCAAGAGTCCTCTTATGTAAGACCGTTGTCAGGTTCCGGTGGGCGTTC5510.10401171877949517No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGACGAGAAGGCACAG5500.10382294977989534No Hit
GCATAAAGGGACTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACATC5400.10193525978389724No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTG5320.10042510778709877No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5300.10004756978789917No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA5300.10004756978789917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)