FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360500
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12510.34701803051317615No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11630.32260748959778085No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11590.32149791955617196No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9930.2754507628294036No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9860.2735090152565881No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG9490.26324549237170597No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT8720.2418862690707351No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8680.2407766990291262No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8630.23938973647711512No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA8340.23134535367545075No Hit
GAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAAC8250.2288488210818308No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACT7970.22108183079056865No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7930.2199722607489598No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7860.21803051317614425No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7800.21636615811373092No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7460.20693481276005546No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7330.20332871012482662No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7150.19833564493758668No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC7030.19500693481276005No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6990.19389736477115116No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6870.19056865464632455No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6630.18391123439667129No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6590.1828016643550624No Hit
GTAGAGTTTCCTTGAGCAGGACTCGTGCAGGTCTTGCATGGTCCCGTGCT6260.17364771151178918No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6250.17337031900138697No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6210.17226074895977808No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6190.17170596393897364No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6030.16726768377253814No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5990.16615811373092926No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5980.16588072122052705No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5980.16588072122052705No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5870.16282940360610262No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5710.15839112343966713No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5670.15728155339805827No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5640.1564493758668516No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5550.1539528432732316No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5420.15034674063800277No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5380.14923717059639388No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5350.14840499306518726No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5310.14729542302357837No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5280.1464632454923717No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA5270.1461858529819695No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGACTTTTCTGTGTC5130.1423023578363384No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5110.14174757281553396No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC5080.14091539528432734No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5070.1406380027739251No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5070.1406380027739251No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5060.1403606102635229No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5040.13980582524271845No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4930.13675450762829405No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4850.13453536754507628No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4830.13398058252427184No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4800.13314840499306518No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4780.13259361997226077No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4770.13231622746185853No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4730.13120665742024964No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4670.12954230235783634No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT4650.1289875173370319No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4640.12871012482662966No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4610.12787794729542304No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4580.12704576976421636No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4560.12649098474341192No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4540.12593619972260747No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4540.12593619972260747No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4490.12454923717059639No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4440.12316227461858531No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4430.12288488210818309No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAAGGGAT4420.12260748959778087No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAAGTCTTGTAAGTTGGCGA4410.12233009708737864No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4320.11983356449375866No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4290.11900138696255202No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4290.11900138696255202No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4280.1187239944521498No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA4260.11816920943134536No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4190.11622746185852982No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4170.11567267683772538No Hit
GTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4150.11511789181692093No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4150.11511789181692093No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA4090.11345353675450763No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4090.11345353675450763No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4040.11206657420249654No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4020.11151178918169209No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4020.11151178918169209No Hit
CTATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA3950.10957004160887657No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3910.10846047156726768No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC3890.10790568654646324No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3870.10735090152565882No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCCCTCAGGCA3870.10735090152565882No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG3830.10624133148404993No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG3820.10596393897364771No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTGTGAGTCCAA3810.10568654646324549No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGC3810.10568654646324549No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3800.10540915395284328No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3770.10457697642163662No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC3760.1042995839112344No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3730.10346740638002773No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC3720.10319001386962551No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC3710.10291262135922331No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA3710.10291262135922331No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG3660.1015256588072122No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCT3640.10097087378640776No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG3620.10041608876560333No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG3620.10041608876560333No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3620.10041608876560333No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3610.10013869625520111No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)