FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516023
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19060.3693633810896026No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA14450.28002627789846574No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14170.2746001631710215No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC13890.2691740484435771No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG13750.26646099107985494No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12780.24766337934549432No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12560.2434000034882166No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12190.23622978045552234No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG11480.22247070382521708No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11060.21433153173405062No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC10020.19417739131782885No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9910.19204570338919003No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT9910.19204570338919003No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9830.1904953848956345No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC9680.18758853772021788No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9610.18623200903835682No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9570.18545684979157906No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9460.18332516186294023No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9450.18313137205124577No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9420.18255000261616247No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9180.1778990471354959No Hit
ATACCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG9100.17634872864194037No Hit
GTATACATCTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC8860.17169777316127383No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8840.17131019353788493No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA8810.17072882410280163No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA8720.16898471579755164No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8560.16588407881044062No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8380.1623958621999407No Hit
GTGCATGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGA8220.15929522521282966No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8120.15735732709588526No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8100.1569697474724964No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT8080.1565821678491075No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7970.15445047992046865No Hit
CATCTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT7880.1527063716152187No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7770.15057468368657986No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7610.1474740466994688No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7480.1449547791474411No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT7420.14379204027727446No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCA7300.1414665625369412No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG7120.13797834592644126No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6960.1348777089393302No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6960.1348777089393302No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6770.13119570251713586No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6770.13119570251713586No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6730.1304205432703581No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6720.13022675345866366No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6620.12828885534171924No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6570.12731990628324705No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6560.1271261164715526No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6560.1271261164715526No Hit
CCCCATCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCACCT6480.12557579797799712No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6370.12344411004935826No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6260.12131242212071941No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAGCCCTGTTCCGA6260.12131242212071941No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6250.12111863230902498No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6150.1191807341920806No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6060.11743662588683061No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6060.11743662588683061No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5940.11511114814649734No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5910.11452977871141404No Hit
GTTGACACAGAAAGGCCTTGTAAGTTGGCGAAAAAGTGAAAGCCTGCTTA5900.11433598889971958No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5870.11375461946463628No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5860.11356082965294181No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5840.11317325002955295No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5830.11297946021785851No Hit
CTCCCCATCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC5800.11239809078277518No Hit
CTCTTTACGCGGTCTCCCCATCTGTGCCTTCTCATCTGCCGGTCCGTGTG5790.11220430097108075No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5690.11026640285413634No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAGATGTATA5680.11007261304244191No Hit
GTATTTGCTGACGCAACCCCCACTGGATGGGGCTTGGCCATAGGCCATCG5670.10987882323074748No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5640.10929745379566415No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5590.10832850473719195No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5580.1081347149254975No Hit
GGAACATATTGTATCAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5510.10677818624363644No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5490.10639060662024755No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATCTGAACCCTAAT5460.10580923718516422No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5420.10503407793838646No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5380.1042589186916087No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5340.10348375944483094No Hit
GGGTAAAGGTGCATGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5330.10328996963313651No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5300.10270860019805318No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTGATACAATA5280.10232102057466431No Hit
GATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC5220.10115828170449766No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5210.10096449189280322No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAAC5180.10038312245771991No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG5180.10038312245771991No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTATTTGCTG5170.10018933264602546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)