FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388110
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA34540.8899538790549071No Hit
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTACATCTCACCATACAG21670.5583468604261679No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21210.5464945505140295No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACATCTCACCATACAG21180.5457215737806292No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC16910.4357012187266497No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGTACCCACG11970.30841771662672957No Hit
TCCCTATACATAGCAGAAGCGGTGTCGAGGAGATCTCGAATGGAAGGAAA11530.29708072453685813No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11200.2885779804694545No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10980.28290948442451885No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10560.2720878101569143No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10440.26899590322331296No Hit
TCCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCT10200.2628120893561104No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9940.25611295766664094No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9420.24271469428770193No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCGTTGACAGACT9410.2424570353765685No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9270.2388498106207003No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA9240.2380768338873No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA8390.21617582644095748No Hit
CCAAAGGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGACA8370.2156605086186906No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC8020.20664244672902013No Hit
ATGTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTACATCTCACCATAC7650.19710906701708278No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7620.19633609028368249No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7610.19607843137254902No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7480.1927288655278143No Hit
CAGTATCTCTTCCAAAAGTAAGACAGGAAAGGTGAAACCACAATAGTTGT7400.19066759423874677No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7390.1904099353276133No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA7270.18731802839401201No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC7150.1842261214604107No Hit
ATCCTAAACTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7110.18319548581587694No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATACTCAAGAA6870.17701167194867434No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6820.17572337739300714No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC6720.17314678828167274No Hit
GTAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6680.17211615263713895No Hit
ATATTAACGTTGACATAGCCGACTACTGCTTCCCTGGATGCTTGGTCTTC6460.16644765659220323No Hit
CAATTTGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT6420.1654170209476695No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA6360.16387106748086883No Hit
GTCCTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATG6360.16387106748086883No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCAGGCGCCGATGACC6340.16335574965860195No Hit
TCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGCGTTT6250.16103681945840098No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6230.16052150163613407No Hit
CCGCTTCTGCTATGTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTACA6210.1600061838138672No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6190.15949086599160034No Hit
TCCTCAGGCCATGCAGTGGAACTCCATAACATTCCACCAGGCTCTGCTAG6160.1587178892582No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT6160.1587178892582No Hit
GAGGTGAAGCGAAGTGCACACGGGCCGGCAGATGAGAAGGCACAGACGGG6150.15846023034706655No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG6050.15588364123573215No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5990.15433768776893148No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5980.15408002885779803No Hit
CTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC5970.1538223699466646No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAGGTAATTTGG5970.1538223699466646No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5790.14918450954626267No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5760.14841153281286232No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5670.14609260261266135No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG5640.14531962587926103No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5590.14403133132359383No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5570.14351601350132695No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5550.14300069567906007No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5520.14222771894565972No Hit
TCTGTGGAGTTGCTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCCATTCG5460.14068176547885908No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5450.14042410656772564No Hit
GCCTTAGAGTCTCCGGAACATTGTACATCTCACCATACAGCACTCAGGCA5440.1401664476565922No Hit
TTGTACATCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG5410.13939347092319188No Hit
GTTCTTGAGTATTTGGTGTCCTTTGGAGTGTGGATTCGCACTCCTCCTGC5410.13939347092319188No Hit
GCTTTAGGGCATGGACATTGACGTGTATAAAGAATTTGGAGCTTCTGTGG5380.13862049418979155No Hit
CACAAATTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAG5350.13784751745639123No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5290.1363015639895906No Hit
GCACGGGGCCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5260.13552858725619027No Hit
GGATGGGGCTTGGCCATAGGTCATCGGCGCCTGCGTGGAACCTTTGTGGC5230.13475561052278992No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5170.13320965705598928No Hit
GTTTCGCCCCAGACCGGCTGCGAGCAAAACACGCTGCTAGGAGTTCCGCA5160.13295199814485584No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT5150.1326943392337224No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5110.13166370358918864No Hit
GGCCTCTACCGTCCCCTTCTTTCTCTGCCGTTCCGGCCGACCACGGGGCG5010.1290871144778542No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG5000.12882945556672076No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4980.12831413774445388No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4980.12831413774445388No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4940.12728350209992012No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4920.12676818427765324No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAAGGTGAAACCACA4920.12676818427765324No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4900.12625286645538636No Hit
GTGTACACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4830.12444925407745226No Hit
GGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG4770.12290330061065162No Hit
GTTGTTAAAATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4760.12264564169951818No Hit
GAGACACTGTTCTTGAGTATTTGGTGTCCTTTGGAGTGTGGATTCGCACT4720.1216150060549844No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTCATTACAATA4720.1216150060549844No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4710.12135734714385096No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4680.12058437041045066No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4670.12032671149931719No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4670.12032671149931719No Hit
TCCATACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCT4630.11929607585478344No Hit
ATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGC4600.1185230991213831No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4570.1177501223879828No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4570.1177501223879828No Hit
GTACTTTACCACAAGAACATATTGTAATGAAAATCAAACAATGTTTTCGG4480.1154311921877818No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4450.1146582154543815No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTG4450.1146582154543815No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTC4440.11440055654324806No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4410.11362757980984772No Hit
CCCTATACATAGCAGAAGCGGTGTCGAGGAGATCTCGAATGGAAGGAAAA4410.11362757980984772No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAAT4370.11259694416531395No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4370.11259694416531395No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4360.11233928525418051No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAGGTAA4340.11182396743191364No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGAC4330.1115663085207802No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT4270.11002035505397956No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4250.10950503723171265No Hit
CCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCA4250.10950503723171265No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4210.1084744015871789No Hit
GCTATGTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTACATCTCACCA4200.10821674267604545No Hit
CTGTAAGAGGGCCCACAAATTGTTCACATCTATTAATAATGTCCTCCTGT4200.10821674267604545No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4190.107959083764912No Hit
GGGAAGCAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTAAGA4170.10744376594264513No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4150.10692844812037826No Hit
CGTTAATATGGGCCTAAAACTAAGACAACTATTGTGGTTTCACCTTTCCT4120.10615547138697792No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGCGTTTG4110.10589781247584448No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCG4060.10460951792017727No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGATTTTCATTACAAT4050.10435185900904383No Hit
AGATAGGGGCATTTGGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACT4020.10357888227564349No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTTAGATCT4010.10332122336451006No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3980.10254824663110974No Hit
GCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGG3930.10125995207544254No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC3910.10074463425317563No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT3900.1004869753420422No Hit
CTTTTTTGCCTTCTGACTTTTTTCCTTCCATTCGAGATCTCCTCGACACC3890.10022931643090877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)