FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439227
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCAACATCTCCTCGACACCGCCTCTGCTCTATATCGGGAGGCCTTAGA26280.5983238735323648No Hit
ATTCGAGATCTCCTCGACACCGCCTCTGCTCTATATCGGGAGGCCTTAGA14500.3301254248941891No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13170.29984495488665314No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12540.2855015743567677No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11450.2606852493129976No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11290.25704248600382035No Hit
CAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG10750.2447481598353471No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA10650.2424714327671113No Hit
GAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGACCCAGC10610.241560741939817No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10450.23791797863063976No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9750.22198088915298922No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9340.2126463081732225No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9020.20536078155486798No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8960.2039947453139265No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8730.19875827305698418No Hit
GTGCTGGTGCACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8700.19807525493651346No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8260.188057655836276No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7990.18191049275203938No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7950.18099980192474505No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTTAGGAAAGGAGGGAGTTTG7850.1787230748565093No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7770.17690169320192065No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC7660.17439729342686128No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7140.1625583126720352No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6790.15458976793320994No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6630.15094700462403268No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6610.15049165921038551No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6580.1498086410899148No Hit
ATATTGTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA6560.14935329567626762No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6530.1486702775557969No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACCGTTAATGAAAAA6490.14775958672850256No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6370.14502751424661964No Hit
GATCTCGAATAGAAGGAAAAAAGTCAGAAGGCAAAAAAGAGAGTAACTCC6210.14138475093744238No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC6130.13956336928285373No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6110.13910802386920657No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6060.1379696603350887No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6050.13774198762826512No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6050.13774198762826512No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5830.13273318807814638No Hit
CTCTTACCGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5800.13205016995767566No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5750.13091180642355776No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5710.13000111559626343No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5630.1281797339416748No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5620.12795206123485123No Hit
GGGTAAAGGTGCAGATATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA5440.12385395251202681No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5420.12339860709837966No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5420.12339860709837966No Hit
GTACTAAAGCTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5390.12271558897790891No Hit
CGGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGC5350.12180489815061461No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5270.11998351649602597No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5260.11975584378920238No Hit
CATTACCTCACCCCAACACAGAAGAGCTTGCCTGAGTGCTGTATGGTGAG5240.11930049837555524No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5150.11725144401414302No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTGCAGG5140.11702377130731945No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5100.11611308048002514No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5070.1154300623595544No Hit
AATTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGATTCAAATGTATA5060.11520238965273082No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5010.11406402611861292No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5010.11406402611861292No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4990.11360868070496577No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4970.11315333529131862No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4970.11315333529131862No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4960.11292566258449505No Hit
TCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGA4940.11247031717084788No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4920.11201497175720072No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4880.1111042809299064No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4810.10951057198214136No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4720.10746151762072916No Hit
CTATTGTGGTTTCACATTTCTTGTCTTACTTTTGGAAGAGAAACTGTTCT4650.1058678086729641No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4650.1058678086729641No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCCTGGTGGAATGTGGTGG4630.10541246325931693No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4630.10541246325931693No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4610.10495711784566977No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4610.10495711784566977No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4590.10450177243202263No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4570.10404642701837545No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4480.10199737265696325No Hit
ATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4460.10154202724331611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)