FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390947
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21400.5473887764837689No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC16150.4130994738417228No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG14910.38138161950341093No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12670.32408485037613793No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT11980.3064353991717547No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10420.26653229210097534No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC9360.23941864242467648No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATA8980.22969865480487123No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8850.22637339588230632No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8770.2243270826991894No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8580.2194670888892868No Hit
GTCTTTGAAGTATGCCTCAAGGTCGATCGTTGACATTGCTGAGAGTCCAA8520.21793235400194913No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8320.21281657104415688No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8250.2110260470089296No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7860.20105027024123473No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7290.18647028881152689No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG7290.18647028881152689No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA7180.18365660818474117No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7130.1823776624452931No Hit
GTGTAAAAGGGGCAGCGAAGCCCAAAAGACCCACAATTCTTTGACATACT7120.18212187329740348No Hit
ATTGACAACAGTGCCAGCAGCCCCTCCTCCTGCCTCCACCAATCGGCAGT7080.18109871670584504No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGGGTTTG7050.1803313492621762No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATATT6980.17854082522694892No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA6890.17623872289594242No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC6790.1736808314170463No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6620.1693324159029229No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6520.16677452442402677No Hit
ACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCC6500.16626294612824757No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA6370.1629376872056826No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAT6210.15884506083944883No Hit
CAGCCATGTCAATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGT6170.15782190424789036No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6130.15679874765633192No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6040.15449664532532542No Hit
GTATACATGCATATAAAGGCATCAACGCAGGATAGCCACATTGTGTAAAA5990.15321769958587736No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5760.1473345491844163No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCACAG5660.14477665770552017No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG5600.14324192281818252No Hit
CTCTTACAATTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5530.14145139878295523No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5510.14093982048717602No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5490.1404282421913968No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5430.1388935073040591No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5410.1383819290082799No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5310.13582403752938377No Hit
ATGTATCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCT5240.1340335134941565No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5220.13352193519837727No Hit
GTATGGATCGGTAGAGGAGCCACAAAGGTTCCACTCAGGCGCCGATGGCC5160.1319872003110396No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5060.12942930883214349No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5040.12891773053636427No Hit
CATATAAAGGCATCAACGCAGGATAGCCACATTGTGTAAAAGGGGCAGCG4990.1276387847969162No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4870.12456931502224088No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4820.12329036928279281No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4820.12329036928279281No Hit
CATGTATACAAACTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4700.12022089950811747No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4700.12022089950811747No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC4700.12022089950811747No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCGAAGCCCAAAAGACCCACAAT4670.11945353206444863No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4670.11945353206444863No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4610.11791879717711097No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4580.11715142973344211No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGGGTTTGCCACTCAGGATTAAAG4580.11715142973344211No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4520.11561669484610447No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4480.11459353825454602No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4460.11408195995876678No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGATCGTTGACA4450.11382617081087718No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACCCCCTCCTTTCCTAAC4430.11331459251509796No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4360.11152406847987066No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4360.11152406847987066No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4320.11050091188831222No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4280.10947775529675377No Hit
GTTTGTATACATGCATATAAAGGCATCAACGCAGGATAGCCACATTGTGT4270.10922196614886416No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4230.10819880955730571No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4100.10487355063474077No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4090.10461776148685115No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4040.10333881574740311No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4020.10282723745162388No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4020.10282723745162388No Hit
CCTCAAGGTCGATCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3990.10205987000795505No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA3970.10154829171217582No Hit
CCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC3920.10026934597272776No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3920.10026934597272776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)