FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485676
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCT17930.36917615859132424No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC16480.33932086411517143No Hit
GGTATAAAGGGCTTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT13840.28496363831031385No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13780.2837282468147489No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT13670.28146336240621317No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT12850.26457967863349224No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12640.260255808399015No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11960.24625470478261227No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAA11850.24398982037407654No Hit
TAAACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCGAGCAG11480.2363715728180927No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9840.20260420527265088No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9810.20198650952486844No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9710.19992752369892686No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9530.19622134921223203No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9090.1871618115780891No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9060.1865441158303066No Hit
ATTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA9050.18633821724771246No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8910.18345563709139426No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8870.18263204276101763No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCGAGTACAATGACC8800.18119075268285853No Hit
CTTTATTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGG8640.177896375361352No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8580.17666098386578707No Hit
ATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8490.17480789662243965No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT8480.1746019980398455No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA8340.1717194178835273No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7860.16183628591900773No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7820.1610126915886311No Hit
GGTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7750.159571401510472No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7690.15833601001490705No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7530.15504163269340054No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC7490.15421803836302392No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7470.1538062411978356No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG7470.1538062411978356No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG7380.1519531539544882No Hit
CTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGT7300.1503059652937349No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7260.14948237096335829No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7150.14721748655482256No Hit
GGGTTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTG7120.14659979080704008No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7090.1459820950592576No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7070.1455702978940693No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7070.1455702978940693No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7050.14515850072888098No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6980.14371721065072188No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA6930.1426877177377511No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6920.14248181915515692No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6590.13568716592954974No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6500.1338340786862023No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6400.13177509286026073No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6320.1301279041995075No Hit
AAGACACAGTCATCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACC6270.1290984112865367No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG6230.12827481695616005No Hit
TCCTTGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA6190.12745122262578346No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6180.12724532404318928No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6120.12600993254762433No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6060.1247745410520594No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6060.1247745410520594No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6030.12415684530427692No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5990.1233332509739003No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5980.12312735239130614No Hit
GGACCATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5970.12292145380871197No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5880.12106836656536457No Hit
GTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5860.12065656940017624No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5860.12065656940017624No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5850.12045067081758208No Hit
GTTGTACCCTGCGTTCGGAGCCAACTCAAACAATCCAGATTGGGACTTCA5820.1198329750697996No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5770.11880348215682884No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5700.11736219207866973No Hit
GTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACT5700.11736219207866973No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA5700.11736219207866973No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5690.11715629349607556No Hit
GGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAG5640.11612680058310479No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5630.11592090200051063No Hit
TTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACAC5560.11447961192235152No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5500.11324422042678658No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG5490.11303832184419242No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5460.11242062609640995No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5400.11118523460084502No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5390.11097933601825086No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5370.11056753885306254No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5340.10994984310528007No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5260.10830265444452682No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAACAATGTCAACGACCGACCT5210.10727316153155601No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5160.10624366861858522No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5160.10624366861858522No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5100.10500827712302029No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5100.10500827712302029No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5070.10439058137523781No Hit
CTTTACCACAGGACCATATTGTACTAAAACTCAAGCAATGTTTTCGGAAA5030.10356698704486117No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4980.10253749413189041No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4920.10130210263632544No Hit
AAGGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT4890.10068440688854297No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4870.10027260972335468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)