FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485676
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCT16700.3438506329322429No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC15420.31749561436019075No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT14110.29052290004035614No Hit
CTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT12860.2647855772160865No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12610.2596381126512325No Hit
GGTATAAAGGGCTTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT12480.25696143107750846No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12310.2534611551734078No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11920.24543111045223562No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG11880.24460751612185905No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGTTGAGAGTCCAA11770.24234263171332326No Hit
TAAACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCGAGCAG9570.19704494354260865No Hit
CTTTATTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGG9560.19683904496001448No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9320.19189747897775472No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9270.19086798606478395No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8830.181808448430641No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8400.17295480937909224No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8260.17007222922277404No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8240.16966043205758571No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA8220.1692486348923974No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCGAGTACAATGACC8150.1678073448142383No Hit
ATTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA8150.1678073448142383No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8090.16657195331867333No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7960.1638952717449493No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7940.163483474579761No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7930.16327757599716686No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG7680.1581301114323129No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7660.1577183142671246No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC7620.15689471993674795No Hit
ATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7530.15504163269340054No Hit
GGTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7460.15360034261524144No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7440.15318854545005312No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7330.1509236610415174No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7140.1470115879722284No Hit
CTCTTATATAGAATGCCCGCCTTCCACAAAGTATGTAAATAATGCCTAGT7100.14618799364185178No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7080.14577619647666346No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA7000.1441290078159102No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA6970.14351131206812773No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6910.14227592057256278No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6790.1398051375814329No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6740.13877564466846212No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6640.13671665884252052No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6640.13671665884252052No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6580.1354812673469556No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6560.13506947018176726No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6480.13342228152101399No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG6470.13321638293841986No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6460.13301048435582571No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6360.1309514985298841No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6050.12456864246946525No Hit
GGGTTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTG6040.12436274388687109No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6040.12436274388687109No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6030.12415684530427692No Hit
GTACAATATGGTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5970.12292145380871197No Hit
GTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACT5870.12086246798277041No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAACAATGTCAACGACCGACCT5860.12065656940017624No Hit
GGACCATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5840.12024477223498793No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5810.11962707648720547No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5810.11962707648720547No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5800.11942117790461132No Hit
GGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAG5780.11900938073942299No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5760.11859758357423468No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGGTGG5640.11612680058310479No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5570.11468551050494567No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5560.11447961192235152No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5560.11447961192235152No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5490.11303832184419242No Hit
GTTGTACCCTGCGTTCGGAGCCAACTCAAACAATCCAGATTGGGACTTCA5460.11242062609640995No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5430.11180293034862747No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5430.11180293034862747No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5390.11097933601825086No Hit
TCCTTGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA5310.10933214735749759No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5260.10830265444452682No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5200.10706726294896186No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5140.10583187145339693No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5130.10562597287080276No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5110.10521417570561445No Hit
GTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5080.10459647995783197No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5080.10459647995783197No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5060.10418468279264365No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5040.10377288562745533No Hit
AAGACACAGTCATCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACC5030.10356698704486117No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAAGGGGCAGCA5000.10294929129707872No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4960.10212569696670208No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4910.10109620405373129No Hit
TTTACATACTTTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACAC4870.10027260972335468No Hit
CTTTACCACAGGACCATATTGTACTAAAACTCAAGCAATGTTTTCGGAAA4870.10027260972335468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)