FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences350361
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20330.5802586475092832No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15890.4535322139165032No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11060.31567440440003314No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA10940.3122493656542823No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10780.30768264732661454No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9070.2588758451996655No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8900.25402370697651855No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8540.24374859073926608No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8180.23347347450201364No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8130.23204637502461747No Hit
ATACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAG7500.21406492160942572No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7410.2114961425501126No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7330.2092127833862787No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7280.20778568390888258No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7200.20550232474504868No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTCTACCACAG7190.20521690484956945No Hit
ACCAAAGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7010.20007934673094324No Hit
GTGGTAGAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6870.19608346819423397No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTACGCGGGACGTCC6820.19465636871683775No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA6690.19094591007560774No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6500.18552293206150228No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6430.18352499279314763No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6350.18124163362931378No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6320.18038537394287607No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6220.1775311749880837No Hit
ATATGTTCCTGTGGTAGAGTACCCCAACTTCCAATTACATATCCCATGAA6200.17696033519712526No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5950.1698248378101444No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5760.16440185979603894No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT5680.16211850063220506No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5600.1598351414683712No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5500.15698094251357886No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5480.15641010272262038No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTACATGTTCAGG5450.1555538430361827No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5450.1555538430361827No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5440.15526842314070344No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5280.1507017048130357No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA5260.15013086502207723No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT5250.14984544512659798No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5190.14813292575372258No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5080.14499330690345102No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4960.1415682681577002No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4960.1415682681577002No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4940.14099742836674173No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4800.13700154983003243No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA4770.13614529014359475No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4690.13386193097976087No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4670.13329109118880242No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4560.13015147233853083No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4420.12615559380182156No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4340.12387223463798766No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4330.12358681474250845No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4330.12358681474250845No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4290.12244513516059151No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4290.12244513516059151No Hit
CCAAAGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACCA4280.12215971526511227No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4260.1215888754741538No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4260.1215888754741538No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4220.12044719589223687No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4220.12044719589223687No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4220.12044719589223687No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4180.11930551631031992No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4170.1190200964148407No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4170.1190200964148407No Hit
CTCTACCACAGGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAA4150.11844925662388223No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT4120.11759299693744452No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG4080.11645131735552759No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4070.11616589746004835No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATTTGGTATTGGGGGC4070.11616589746004835No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4060.11588047756456912No Hit
TACTTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3990.11388253829621449No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3990.11388253829621449No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG3980.11359711840073525No Hit
ATACCAAATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3940.11245543881881831No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT3930.11217001892333907No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA3900.11131375923690136No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3870.11045749955046368No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT3870.11045749955046368No Hit
GTCTTTAAATAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3770.10760330059567133No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG3740.10674704090923362No Hit
GTACAGACTTGGCCCCCAATACCAAATCATCCATATAACTGAAAGCCAAA3730.10646162101375439No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3720.10617620111827515No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3690.10531994143183744No Hit
GCACTCAGATGCCGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG3690.10531994143183744No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3680.1050345215363582No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC3660.10446368174539976No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3630.10360742205896205No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTCCCTAAC3520.10046780320869048No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)