FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576672
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21520.37317573941512683No Hit
CCTAATCTCCTCCCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA18510.32097969036124524No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16060.2784945341545974No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA15900.27571999334110203No Hit
ATATAAGAGAGAATCTACACGTAGCGCCTCATTTTGTGGGTCACCATATT15820.27433272293435434No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAGAGACCCACAATTCTCTGACATACT14940.25907274846012984No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14660.254217302036513No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC13110.22733893790577658No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12910.2238707618889074No Hit
CTATATAAGAGAGAATCTACACGTAGCGCCTCATTTTGTGGGTCACCATA12780.22161644747794243No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCCTAT11460.19872648576660562No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11370.19716580655901447No Hit
CAAAACACCTCTATGTTTCACTCTTGTTGCTGTACAAAACCTTCGGACGG11170.19369763054214525No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA11110.19265717773708452No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAATCTACA11010.1909230897286499No Hit
GGTTCCTTGAGCAGGATTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG10500.18207924088563343No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10480.1817324232839465No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10390.18017174407635536No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10100.175142888851895No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9620.16681926641140893No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9590.16629904000887852No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT9580.16612563120803508No Hit
CAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG9400.16300427279285276No Hit
CATTTAAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9350.16213722878863548No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG9330.16179041118694856No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9290.16109677598357472No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9250.16040314078020088No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8950.15520087675489708No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8560.1484379335220021No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC8520.14774429831862826No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8490.1472240719160979No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8410.1458368015093502No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8260.1432356694966983No Hit
CCTAGTCTCCTCCCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA8250.14306226069585484No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTGCGGAAAGGAGGGAGTTTG8210.142368625492481No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8090.14028771988235947No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7830.1357790910604295No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG7820.13560568225958602No Hit
GGATAATAGGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7810.1354322734587426No Hit
CTTTTGGAGTGTGGATTCGCACCCCTCCTGCTTACAGACCACCAAATGCC7800.1352588646578991No Hit
CCCCTAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTACGCCTCA7650.13265773264524722No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7600.1317906886410299No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7490.12988319183175184No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCCAATAAAACCAAACGTTGGG7380.12797569502247377No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7320.126935242217413No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7170.12433411020476111No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7150.12398729260307419No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG7120.1234670662005438No Hit
GGTGTTTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG7020.1217329781921092No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6970.1208659341878919No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAATCTACAC6960.12069252538704844No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6890.11947866378114423No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6870.11913184617945731No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6790.11774457577270962No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6710.11635730536596192No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC6590.11427639975584042No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6420.11132845014150158No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA6400.11098163253981466No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG6360.11028799733644082No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCGCAC6320.10959436213306697No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCGCACATTCATTTACAGG6260.10855390932800622No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6250.10838050052716275No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6240.1082070917263193No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6220.10786027412463238No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6190.10734004772210198No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6140.10647300371788469No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG6130.10629959491704123No Hit
TCTTATGTCTTTGGGTATACATTTAAACCCCAATAAAACCAAACGTTGGG6070.10525914211198047No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG6070.10525914211198047No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6060.10508573331113699No Hit
ATGTTAATATGGGCCTAAAGATCAGACAACTACTGTGGTTTCACATTTCC6000.10404528050607625No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5990.10387187170523278No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5940.10300482770101549No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5940.10300482770101549No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5920.10265801009932855No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC5890.10213778369679818No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5870.10179096609511126No Hit
GTTCAGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG5870.10179096609511126No Hit
CAAGGAACCTCTATGTTTCACTCTTGTTGCTGTACAAAACCTTCGGACGG5860.10161755729426779No Hit
GGTGTTTTGAGCAGGATTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAG5860.10161755729426779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)