FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542170
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACAGAGTATGTAAATAATGCCTAGCTTTGAAGTAATGATTAACTACC33010.6088496228120331No Hit
CTCTATGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCT31710.5848719036464578No Hit
GCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT17700.3264658686389878No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCT17520.3231458767545235No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16570.30562369736429534No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA15830.2919748418392755No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA15580.2873637419997418No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15320.2825681981666267No Hit
ACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA15230.28090820222439455No Hit
TCCATAGAGTATGTAAATAATGCCTAGCTTTGAAGTAATGATTAACTACC14480.2670749027057934No Hit
CCTCCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14260.26301713484700373No Hit
ATTGATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAAAG14000.25822159101388864No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC13350.24623273143110094No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG12780.23571942379696403No Hit
CCTATCAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTT12490.2303705479831049No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAAAG11950.2204105723297121No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGG11380.20989726469557518No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11300.20842171274692442No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT10910.20122839699725178No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10860.20030617702934506No Hit
GCCTTTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT10470.19311286127967242No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTT10230.18868620543372006No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10150.18721065348506924No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAA10120.1866573215043252No Hit
GGCCAAAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACC10050.18536621354925578No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9950.1835217736134423No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTGAAA9890.18241510965195418No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCGCAG9810.1809395577033034No Hit
GTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG9740.17964844974823396No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9720.17927956176107127No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC8950.16507737425530739No Hit
ATGTAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTCCATGC8950.16507737425530739No Hit
GTATGTAAATAATGCCTAGCTTTGAAGTAATGATTAACTACCTGTTCAGG8840.16304849032591254No Hit
CTATCAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG8680.16009738642861096No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT8600.15862183447996014No Hit
GCCAAAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACCC8590.1584373904863788No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA8490.15659295055056532No Hit
ATTTACAGGCAGTTTCCGAAAACATTTCTTGATTTTCAGTACAATATGTT8400.1549329546083332No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8190.15105963074312487No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT8170.15069074275596214No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG8120.1497685227880554No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7930.14626408691000978No Hit
CTGTACGGTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC7780.14349742700628954No Hit
GCTCTCTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA7690.1418374310640574No Hit
ATATTAACGTTGACATAGCTGACTACTAAGTCCCTGGATGCTTGATCTTC7690.1418374310640574No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7640.14091521109615066No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGATTTGATGA7590.1399929911282439No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTGAAAGGGGCAGCA7510.1385174391795931No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7390.13630411125661693No Hit
CCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC7350.13556633528229153No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT7340.13538189128871017No Hit
ATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTGCCACTC7280.13427522732722208No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGA7250.13372189534647805No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAAGCGCTGCGT7160.1320618994042459No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7150.13187745541066456No Hit
CTGTAAGGTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC7130.13150856742350187No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7110.13113967943633914No Hit
GCCCCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT7090.13077079144917647No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG6970.1285574635262003No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG6880.12689746758396814No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6870.12671302359038678No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA6850.12634413560322408No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6800.12542191563531735No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6780.12505302764815462No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6780.12505302764815462No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6770.12486858365457329No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATGTTCG6730.12413080768024788No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6640.12247081173801576No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6620.12210192375085305No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC6600.12173303576369035No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC6470.11933526384713283No Hit
GTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCCCA6450.11896637585997012No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA6400.11804415589206337No Hit
GGGTTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6390.11785971189848202No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6360.11730637991773799No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTGCCAATTACATATCCC6300.11619971595624988No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6240.11509305199476179No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6180.11398638803327368No Hit
CTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGCACTGT6160.113617500046111No Hit
GTGTGAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6160.113617500046111No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG6150.11343305605252965No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6050.11158861611671617No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA6030.11121972812955347No Hit
TCCAGGGACTTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAAT6030.11121972812955347No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6030.11121972812955347No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC6000.11066639614880941No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5990.11048195215522807No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5980.11029750816164673No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5960.10992862017448402No Hit
GGCCAGAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACC5910.10900640020657726No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5870.10826862423225188No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5870.10826862423225188No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATCAATAATGTCCTCCTGT5830.10753084825792648No Hit
CTTATATAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTCCA5820.10734640426434514No Hit
TTGTACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG5750.10605529630927568No Hit
CGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC5750.10605529630927568No Hit
GTGTTGGGGTGATTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5710.1053175203349503No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGATTTGATGAATCTG5660.10439530036704354No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5650.10421085637346221No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCCTCCTTTCCTAAT5630.1038419683862995No Hit
GTCGTAGTTTGGTGGAATGTGGTGGAGTTCCACTGCATGGCCTGAGGATG5620.10365752439271815No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5580.10291974841839274No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5550.10236641643764871No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5500.10144419646974197No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA5490.10125975247616062No Hit
CCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAAC5430.10015308851467253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)