FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542170
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCACAGAGTATGTAAATAATGCCTAGCTTTGAAGTAATGATTAACTACC31880.5880074515373407No Hit
CTCTATGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCT30470.5620008484423704No Hit
CTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACGCAGCGCT18090.3336591843886604No Hit
GCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT16450.3034103694413191No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15710.28976151391629934No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15300.282199310179464No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA14420.2659682387443053No Hit
TCCATAGAGTATGTAAATAATGCCTAGCTTTGAAGTAATGATTAACTACC13990.2580371470203073No Hit
ATTGATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAAAG13830.2550860431230057No Hit
CCTCCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13780.25416382315509894No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC13560.25010605529630925No Hit
ACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA12710.23442831584189458No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11580.21358614456720215No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11580.21358614456720215No Hit
ATTAATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAAAG11400.2102661526827379No Hit
CCTATCAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTT11110.20491727686887876No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT10960.20215061696515854No Hit
GTCTAGACTTGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG10460.1929284172860911No Hit
CCTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTT10460.1929284172860911No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10070.18573510153641848No Hit
TTATTAATAGGTGTCAACAATATGTGGGCCCTCTCACAGTTAATGAAAAA10040.18518176955567442No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9830.18130844569046609No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCGCAG9830.18130844569046609No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGG9790.1805706697161407No Hit
GCCTTTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT9760.18001733773539663No Hit
GGCCAAAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACC9750.1798328937418153No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTGAAA9690.1787262297803272No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9140.168581810133353No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC8860.16341737831307523No Hit
GTATGTAAATAATGCCTAGCTTTGAAGTAATGATTAACTACCTGTTCAGG8690.1602818304221923No Hit
ATTTACAGGCAGTTTCCGAAAACATTTCTTGATTTTCAGTACAATATGTT8600.15862183447996014No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8550.15769961451205342No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8400.1549329546083332No Hit
CTATCAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG8220.1516129627238689No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG7900.14571075492926575No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT7900.14571075492926575No Hit
ATATTAACGTTGACATAGCTGACTACTAAGTCCCTGGATGCTTGATCTTC7790.1436818709998709No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7730.14257520703838278No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATGTTCG7590.1399929911282439No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT7580.13980854713466256No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7580.13980854713466256No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7560.13943965914749987No Hit
GCCAAAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACCC7470.13777966320526772No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC7400.13648855525019826No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7400.13648855525019826No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7310.13482855930796614No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7210.13298411937215265No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7150.13187745541066456No Hit
GTATCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAAGCGCTGCGT7150.13187745541066456No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT7100.1309552354427578No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA7100.1309552354427578No Hit
GCCCCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTT7040.12984857148126971No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7010.12929523950052565No Hit
GCTCTCTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA6970.1285574635262003No Hit
ATGTAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTCCATGC6950.12818857553903756No Hit
CTGTACGGTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC6930.12781968755187487No Hit
CTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGCTGCTGGCACTGT6920.12763524355829353No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6780.12505302764815462No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6710.1237619196930852No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTGCCAATTACATATCCC6700.12357747569950385No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG6690.1233930317059225No Hit
CCTTTACCCCGTTGCACGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6550.12081081579578361No Hit
CGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC6530.12044192780862092No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6450.11896637585997012No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTGAAAGGGGCAGCA6400.11804415589206337No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG6370.11749082391131933No Hit
CCACCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC6360.11730637991773799No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6350.11712193592415662No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGATTTGATGA6300.11619971595624988No Hit
ATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTGCCACTC6260.1154619399819245No Hit
CTGTAAGGTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC6200.11435527602043641No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6180.11398638803327368No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6170.11380194403969235No Hit
GTGTGAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6110.11269528007820426No Hit
TTGTACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG6050.11158861611671617No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC5990.11048195215522807No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5990.11048195215522807No Hit
GTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCCCA5960.10992862017448402No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCACGGCAACGGTCAGGTC5910.10900640020657726No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5860.10808418023867052No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5850.10789973624508917No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5850.10789973624508917No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGATTTGATGAATCTG5830.10753084825792648No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG5820.10734640426434514No Hit
GTGTTGGGGTGATTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5800.10697751627718242No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5780.10660862829001974No Hit
TCCAGGGACTTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAAT5730.10568640832211298No Hit
CTGTAAGAGGGCCCACATATTGTTGACACCTATCAATAATGTCCTCCTGT5680.10476418835420624No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA5670.1045797443606249No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5670.1045797443606249No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5650.10421085637346221No Hit
GGGTTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5640.10402641237988083No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5630.1038419683862995No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5610.1034730803991368No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5590.10310419241197412No Hit
GGCCAGAGGCAAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACC5540.10218197244406736No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5530.101997528450486No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5500.10144419646974197No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5460.10070642049541656No Hit
GTCGTAGTTTGGTGGAATGTGGTGGAGTTCCACTGCATGGCCTGAGGATG5430.10015308851467253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)