FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476158
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22660.47589245586549006No Hit
CCTATAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT20580.43220947668631005No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC16940.355764263122745No Hit
CCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTC16810.3530340769240462No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15390.3232120430613368No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG13910.29212992326076637No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG13690.2875096081552762No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12980.27259859122392144No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12950.271968548254991No Hit
GGATATATCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC12660.265878132888663No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA12600.26461804695080204No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT11510.24172648574632788No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA11060.2322758412123707No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC10490.22030502480269157No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT10310.21652476698910866No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10140.21295452349850258No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9870.20728413677812826No Hit
GACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG9690.2035038789645454No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9690.2035038789645454No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTACTGGTGGTTGATGTTCCTGG9520.19993363547393933No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9460.19867354953607835No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9160.19237311984677355No Hit
TCCTCACAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTT9010.18922290500212113No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA8920.1873327760953297No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA8760.1839725469277005No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG8750.18376253260472364No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8730.18334250395877No Hit
TGTCAAGGCAGGTCCTCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAG8620.1810323464060249No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8350.17536195968565055No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8340.17515194536267373No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8340.17515194536267373No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7950.16696138676657749No Hit
GGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC7930.16654135812062382No Hit
GATATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAAT7870.16528127218276287No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7640.16045094275429583No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC7600.15961088546238852No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7360.1545705417109447No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC7300.15331045577308372No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA7280.15289042712713008No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7260.1524703984811764No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6990.1468000117608021No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6810.1430197539472192No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA6750.14175966800935824No Hit
GTTCAGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6620.1390294818106595No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6450.1354592383200534No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC6400.13440916670516928No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6360.13356910941326197No Hit
TTGTTAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGCAGG6330.1329390664443315No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG6310.13251903779837787No Hit
GCCACGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC6300.13230902347540102No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG6260.1314689661834937No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG6190.12999886592265592No Hit
ATTGACAACAGTGCCAGCTGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6030.12663863675502668No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5980.12558856514014255No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5930.12453849352525842No Hit
TGTCAAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAG5860.12306839326442065No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG5840.12264836461846698No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC5820.12222833597251331No Hit
CTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCC5820.12222833597251331No Hit
ATATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5810.1220183216495365No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5790.12159829300358285No Hit
GACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTTAG5790.12159829300358285No Hit
CCTCCCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5670.11907812112786091No Hit
GTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC5660.11886810680488409No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5660.11886810680488409No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5650.11865809248190727No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCTG5620.11802804951297678No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC5610.11781803518999996No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC5590.1173980065440463No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5580.11718799222106947No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5570.11697797789809265No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA5550.116557949252139No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA5530.11613792060618534No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5490.11529786331427805No Hit
GATATATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTTAG5480.1150878489913012No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT5470.11487783466832438No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5410.1136177487304634No Hit
CTAAATCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCC5400.11340773440748658No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5320.11172761982367198No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5290.11109757685474149No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5280.11088756253176467No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC5260.110467533885811No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5260.110467533885811No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5240.11004750523985736No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5220.10962747659390369No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5180.1087874193019964No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5180.1087874193019964No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5170.10857740497901958No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5160.10836739065604274No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5130.10773734768711227No Hit
ATCCTGCTCTGATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTT5120.10752733336413545No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5050.10605723310329765No Hit
ATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATTTACAT5030.10563720445734398No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT4980.10458713284245985No Hit
GTCAATATCTTATCAACACCTCCGGAAACTACTGTTGTTAGACGACGAGG4940.10374707555055256No Hit
GAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTCCCGATAAGTTT4920.10332704690459889No Hit
GCTTTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4900.10290701825864525No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4890.1026970039356684No Hit
GGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA4860.10206696096673794No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4830.10143691799780745No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4810.1010168893518538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)