FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476158
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22580.4742123412816754No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16310.34253336077520485No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC15160.31838171363286977No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG13940.29275996622969686No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC13290.2791090352362031No Hit
CCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTC13130.2757488060685739No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG12440.2612578177831728No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12120.25453735944791434No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11830.24844694408158638No Hit
GGATATATCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC11580.2431965860071657No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT11310.2375261992867914No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA11300.23731618496381454No Hit
CCTATAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT10990.2308057409515329No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA10270.21568470969720138No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA9910.2081241940700356No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9560.20077369276584664No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT9480.199093578182032No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9370.1967834206292869No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA9300.19531332036844912No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG9060.1902729766170053No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8930.18754279041830652No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8650.18166238937495538No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA8550.1795622461451871No Hit
GACACATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAG8480.17809214588434932No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8390.17620201697755788No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8340.17515194536267373No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTACTGGTGGTTGATGTTCCTGG8230.17284178780992862No Hit
TCCTCACAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTT8200.17221174484099816No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8160.17137168754909085No Hit
GGATGTGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGC8070.1694815586422994No Hit
TGTCAAGGCAGGTCCTCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAG8000.16801145838146161No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7900.16591131515169333No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA7430.15604064197178247No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7340.15415051306499103No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC7250.1522603841581996No Hit
GATATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAAT7160.15037025525140815No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7080.14869014066759353No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6760.14196968233233506No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA6720.14112962504042775No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6620.1390294818106595No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6530.13713935290386806No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC6500.13650930993493757No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6260.1314689661834937No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6240.13104893753754007No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG6230.13083892321456322No Hit
ATTGACAACAGTGCCAGCTGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6060.12726867972395717No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG5920.12432847920228159No Hit
GTTCAGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5900.12390845055632795No Hit
CCTCCCGCCTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5900.12390845055632795No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5890.12369843623335111No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG5860.12306839326442065No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5800.12180830732655967No Hit
CTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCC5770.12117826435762918No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG5740.1205482213886987No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCTG5700.1197081640967914No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5670.11907812112786091No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5670.11907812112786091No Hit
ATATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5560.11676796357511582No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5540.11634793492916216No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC5500.11550787763725487No Hit
GCCACGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC5480.1150878489913012No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5470.11487783466832438No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5400.11340773440748658No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5380.11298770576153294No Hit
TTGTTAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGCAGG5310.11151760550069516No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5300.11130759117771831No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5280.11088756253176467No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5270.11067754820878785No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5260.110467533885811No Hit
TGTCAAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAG5260.110467533885811No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5230.10983749091688054No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5210.10941746227092687No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA5200.10920744794795005No Hit
TGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC5200.10920744794795005No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5170.10857740497901958No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATT5170.10857740497901958No Hit
GGGTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA5160.10836739065604274No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5140.10794736201008909No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5070.10647726174925129No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5040.10584721878032083No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4930.10353706122757574No Hit
GTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGC4870.10227697528971476No Hit
GATATATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTTAG4870.10227697528971476No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4840.10164693232078427No Hit
ATCCTGAACATGCAGTTAATCATTACTTTAAAACTAGGCATTATTTACAT4840.10164693232078427No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4810.1010168893518538No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4810.1010168893518538No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4800.10080687502887697No Hit
GTCAATATCTTATCAACACCTCCGGAAACTACTGTTGTTAGACGACGAGG4790.10059686070590015No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT4770.10017683205994649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)