FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550219
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC25470.4629065881040095No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17120.31114883346449324No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15220.27661712881598055No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13750.2499004941668681No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13500.2453568488183796No Hit
ATGTAAGACCTTGGGCAGGACCTGGTGGGCGTTCACGGTGGTTTCCATGC12280.22318385951775566No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG12130.22045767230866256No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT12020.2184584683553276No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11590.21064339835592735No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11050.2008291244031922No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT11050.2008291244031922No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10840.19701246231046185No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10710.1946497667292478No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10540.1915600878922756No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10540.1915600878922756No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10500.19083310463651745No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT10380.18865215486924297No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10080.18319978045105678No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9680.17592994789347513No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG9320.16938709859165169No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8630.1568466374298234No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8380.15230299208133488No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT8310.15103077138375812No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8300.15084902556981855No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8160.148304584174665No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7930.14412443045405557No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG7930.14412443045405557No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7920.14394268464011603No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7900.14357919301223693No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC7800.14176173487284155No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7800.14176173487284155No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7700.13994427673344614No Hit
CCTTTACCCCGTTGCCCGACAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7520.1366728520825344No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7510.13649110626859487No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7440.1352188855710181No Hit
ATTTACATACTCTGTGGAAAGCTGGCATTCTATATAAAAGAGAAACTACA7440.1352188855710181No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA7340.13340142743162267No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT7300.13267444417586452No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7240.1315839692922273No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7230.13140222347828773No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7190.13067524022252958No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC7170.1303117485946505No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG7130.12958476533889235No Hit
GTGCTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG7090.12885778208313417No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG7040.12794905301343645No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6960.12649508650192015No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6950.12631334068798059No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6740.12249667859525026No Hit
CCCCAACACAGAATAGCTTGCCTGAGGGCTGTATGGTGAGGTGAACAATG6600.11995223720009669No Hit
ATATAAGACCTTGGGCAGGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6530.1186800165025199No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6480.11777128743282221No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6480.11777128743282221No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6430.11686255836312451No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6380.1159538292934268No Hit
CTTATATAAGACCTTGGGCAGGACCTGGTGGGCGTTCACGGTGGTTTCCA6370.11577208347948727No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6340.11522684603766864No Hit
GGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCT6220.11304589627039414No Hit
GGGTAAAGGTGCAGGTATTGTTGACGCAGAAAGGCCTTGTAAGTTGGCGA6190.11250065882857554No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6190.11250065882857554No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCGTCC6160.1119554213867569No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6150.11177367557281737No Hit
CACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGT6130.1114101839449383No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6060.11013796324736151No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT6040.10977447161948242No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6040.10977447161948242No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6010.1092292341776638No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6000.10904748836372427No Hit
GTGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGG6000.10904748836372427No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5980.10868399673584518No Hit
GTGGAAAGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCTTCAT5960.10832050510796609No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5940.10795701348008703No Hit
ACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC5910.1074117760382684No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG5900.10723003022432885No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5890.10704828441038931No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5880.10686653859644976No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5810.105594317898873No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5780.10504908045705437No Hit
TTTACATACTCTGTGGAAAGCTGGCATTCTATATAAAAGAGAAACTACAC5780.10504908045705437No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5750.10450384301523576No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5710.10377685975947759No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGCCCGGCAGATGAGAAGGCACAG5680.10323162231765898No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA5640.1025046390619008No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5580.10141416417826356No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5540.1006871809225054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)