FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550219
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC26790.48689703554402886No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18870.3429543509039128No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15500.28170601160628767No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13160.2391774911444352No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12670.23027194626139774No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11850.21536878951835542No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT11580.21046165254198784No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11530.20955292347229013No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11430.20773546533289475No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11380.20682673626319703No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11350.2062814988213784No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11200.2035553116122853No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11040.20064737858925263No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10850.19719420812440136No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10840.19701246231046185No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG10780.19592198742682457No Hit
ATGTAAGACCTTGGGCAGGACCTGGTGGGCGTTCACGGTGGTTTCCATGC10730.19501325835712688No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT10600.19265056277591286No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC9990.18156406812560089No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG9870.1793831183583264No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9820.17847438928862872No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9330.16956884440559122No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8900.161753774406191No Hit
CTTCATCTGCCGTTCCAGCCGACCACGGGGCGCACCTCTCTTTACGCGGT8600.15630139998800477No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8380.15230299208133488No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG8360.1519395004534558No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8180.14866807580254407No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8050.14630538022133005No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7950.14448792208193464No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7890.14339744719829742No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG7860.14285220975647878No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7630.13867205603586935No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7570.13758158115223212No Hit
ATTTACATACTCTGTGGAAAGCTGGCATTCTATATAAAAGAGAAACTACA7490.1361276146407158No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT7480.13594586882677626No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT7450.13540063138495764No Hit
CCTTTACCCCGTTGCCCGACAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7420.134855393943139No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA7290.13249269836192498No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7220.1312204776643482No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC7130.12958476533889235No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC7080.12867603626919463No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6960.12649508650192015No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6960.12649508650192015No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG6900.1254046116182829No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6840.12431413673464566No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6820.1239506451067666No Hit
GTGCTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG6810.12376889929282704No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6780.12322366185100841No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6770.12304191603706886No Hit
CCCCAACACAGAATAGCTTGCCTGAGGGCTGTATGGTGAGGTGAACAATG6640.12067922045585484No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6620.12031572882797578No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6600.11995223720009669No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6590.11977049138615714No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6550.11904350813039899No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT6510.11831652487464081No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6350.11540859185160818No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6310.11468160859585003No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6310.11468160859585003No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6250.11359113371221279No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6240.11340938789827323No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC6100.11086494650311966No Hit
ACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC6060.11013796324736151No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6060.11013796324736151No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6030.10959272580554288No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC5990.10886574254978473No Hit
GTGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGG5990.10886574254978473No Hit
GGGTAAAGGTGCAGGTATTGTTGACGCAGAAAGGCCTTGTAAGTTGGCGA5850.10632130115463116No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5810.105594317898873No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5800.10541257208493346No Hit
GTGGAAAGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCTTCAT5800.10541257208493346No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCGTCC5600.10177765580614265No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5570.10123241836432402No Hit
GTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC5560.1010506725503845No Hit
GGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCT5560.1010506725503845No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCCCTCAGGCA5550.10086892673644494No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5520.10032368929462632No Hit
CACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGT5510.10014194348068678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)