FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579406
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18400.3175666113226304No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC17270.2980638792142297No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA16790.2897795328319003No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG15470.26699758028049414No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14810.2556066040047911No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA14680.2533629268595769No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13870.23938309233939586No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG13580.2343779664000718No Hit
CTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG13090.22592102946811043No Hit
GTGTCAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC12990.2241951239717918No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12980.22402253342215994No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12680.218844816933204No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG12280.2119411949479294No Hit
GAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGAGGTG12030.20762643120713284No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTACGCGGGACGTCC11850.20451980131375927No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11830.20417462021449553No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11760.20296648636707249No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC11740.20262130526780875No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC11720.20227612416854504No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA11350.19589027383216606No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11330.1955450927329023No Hit
ATTCACAACAGTGCCAACAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11060.19088514789284197No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT10900.18812369909873217No Hit
ACACAAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA10580.1826008015105125No Hit
CAACACAAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG10560.18225562041124876No Hit
CTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA10450.18035712436529824No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9920.17120982523480946No Hit
GTTGTGAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9880.170519463036282No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG9730.16793060479180402No Hit
CAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG9690.16724024259327655No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT9630.16620469929548537No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9580.16534174654732606No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT9560.16499656544806232No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC9410.16240770720358436No Hit
CCAATAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCAC9160.15809294346278777No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG9160.15809294346278777No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG9060.1563670379664691No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8950.1544685419205186No Hit
AACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT8760.15118932147751318No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8600.14842787268340335No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT8540.14739232938561214No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCGG8480.14635678608782096No Hit
GGGCACATTCACAACAGTGCCAACAGCGCCTCCTCCTGCCTCCACCAATC8460.14601160498855725No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8390.14480347114113418No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGCGGCCG8330.143767927843343No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8330.143767927843343No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA8300.14325015619444742No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8250.1423872034462881No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8090.13962575465217827No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA8080.1394531641025464No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG8060.13910798300328267No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGTACCTCTCTTTACGCGGT7780.13427544761359045No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7710.13306731376616743No Hit
AACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT7670.13237695156763996No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCGGACTTCTTTCCTTCTATTCG7670.13237695156763996No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA7600.1311688177202169No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT7540.13013327442242573No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7460.1287525500253708No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7300.12599110123126098No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7280.12564592013199727No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT7260.12530073903273353No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7260.12530073903273353No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7250.12512814848310166No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7200.12426519573494235No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC7180.1239200146356786No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA7170.12374742408604675No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGGAAGGAGGGAGTTTG7110.12271188078825557No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7060.12184892804009623No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7030.12133115639120064No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7030.12133115639120064No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA6960.12012302254377759No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6900.1190874792459864No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6830.11787934539856336No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6790.1171889832000359No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6740.11632603045187659No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA6700.11563566825334912No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6700.11563566825334912No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6670.11511789660445353No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG6480.1118386761614481No Hit
TGGAAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATAAG6470.11166608561181625No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6410.11063054231402507No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA6320.10907722736733828No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6260.1080416840695471No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTAC6240.10769650297028335No Hit
GACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCA6200.10700614077175591No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6140.1059705974739647No Hit
ACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTC6110.10545282582506911No Hit
ATCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACA6090.10510764472580539No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6070.10476246362654167No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6050.10441728252727793No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6020.10389951087838234No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5990.10338173922948674No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT5970.10303655813022301No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5930.10234619593169555No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5930.10234619593169555No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5900.10182842428279996No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5890.10165583373316811No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG5880.10148324318353624No Hit
CTCCAAAAGACACCAAGTACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5870.10131065263390437No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5830.10062029043537693No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC5830.10062029043537693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)