FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530953
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38410.7234161969138511No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10610.199829363427648No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACTTT10480.19738093578904348No Hit
CTTTTATATAAAATCCCTGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10430.19643923285111867No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9550.1798652611436417No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATACATAAACC9130.1719549564650732No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGA8830.16630473883752422No Hit
GTATAATATGGTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC8410.15839443415895568No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8280.15594600652035112No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT8230.1550043035824263No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAAAGTCCAA7830.1474706800790277No Hit
CTCCAAAAGACACCAGATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7720.14539893361559308No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7700.14502225244042316No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT7650.14408054950249832No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC7300.13748862893702454No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7200.1356052230611749No Hit
ATATGGTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7120.13409849836049517No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6630.1248698095688319No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG6560.12355142545573713No Hit
GTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGTGTTTCTTCAAAA6520.12279806310539727No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5960.11225099020063924No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5700.10735413492343013No Hit
ATATGCAGGTGGAGTGCGAATCCACACTCCAAAAGACACCAGATATTCAA5610.10565906963516546No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA5420.10208059847105111No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5390.10151557670829621No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5310.1000088520076165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)