FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530953
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38190.7192727039869818No Hit
CTTTTATATAAAATCCCTGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9690.18250202936983123No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9410.1772284929174522No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACTTT9400.17704015232986725No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATACATAAACC8080.15217919476865185No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8070.15199085418106686No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7260.1367352665866847No Hit
GTATAATATGGTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCC7250.13654692599909973No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGA7130.13428683894808013No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6950.13089670837155076No Hit
CTATATCACAATGTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGT6790.12788325897019134No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT6730.12675321544468154No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC6650.12524649074400182No Hit
CTCCAAAAGACACCAGATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6560.12355142545573713No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAAAGTCCAA6250.11771286724060323No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG5950.11206264961305427No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5800.10923754079927978No Hit
GTACTTAATCACCTGTTCATGAACATTTCGTTTCCAGTGTTTCTTCAAAA5730.10791915668618504No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5670.10678911316067524No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5540.1043406855220707No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5390.10151557670829621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)