FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417633
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTAGATGTTCAGG26890.643866744246743No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18420.44105710037281537No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT16230.3886187154750702No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG15230.36467424748523225No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT13750.32923643486027204No Hit
GTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTT12830.3072075243096211No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT12220.29260139883582No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG11900.2849391690790718No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11050.26458637128770957No Hit
CTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT10190.24399412881644889No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT10130.24255746073705864No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAAC9950.23824745649888776No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9720.23274022886122506No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTATGGTT9360.22412022038488338No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT8700.20831687151159034No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8600.20592242471260655No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC8590.20568298003270816No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8390.20089408643474055No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC8190.19610519283677297No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT7690.18413295884185396No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA7680.1838935141619556No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7520.18006239928358153No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC7170.17168183548713822No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7050.16880849932835768No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6880.16473793977008522No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAATACAAAGATC6860.16425905041028846No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAACTATT6540.15659682065354033No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTGTATTCTTCTACTGTACCTGTC6460.15468126321435327No Hit
TCCACAAAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTAGA6430.15396292917465815No Hit
CCTCAAGCTTACAGACCACCAAATACTCAAGAACAGTTTCTCTTCCAAAA6090.14582181005811323No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6030.14438514197872293No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5650.1352862441425845No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAA5550.1328917973436007No Hit
ATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTTGGA5550.1328917973436007No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT5490.13145512926421046No Hit
GTTTGGAAGTAATGATTAACTAGATGTTCAGGATAATAAGGTTTGATGCC5300.12690568034614125No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5260.1259479016265477No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5220.12499012290695419No Hit
ATACCGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATTTG5100.12211678674817364No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTGTATTCTTCTACTG5000.11972233994918983No Hit
ATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTTGGAAG4910.11756733783010442No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4790.11469400167132386No Hit
GTGCTGGTGTTTGTGTGTATAATTGAATATTATTCAGCTTTTGTAAAGAA4740.11349677827183197No Hit
CTCCCAATACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG4690.11229955487234006No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATG4670.11182066551254331No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC4470.10703177191457572No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTGTATTC4400.10535565915528707No Hit
GCTTAGCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACAT4240.10152454427691299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)