FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417633
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTAGATGTTCAGG31020.7427573970447737No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18280.43770487485423804No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT13800.33043365825976395No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG13790.33019421357986556No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT13680.3275603221009834No Hit
GTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTT12200.2921225094760232No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT11480.27488249252333985No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11470.27464304784344146No Hit
GGATATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG11140.266741373406795No Hit
CTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT10860.2600369223696403No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATCAAAC10710.2564452521711646No Hit
CTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT10200.24423357349634728No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9970.23872634585868452No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTATGGTT9340.22364133102508663No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8850.211908541710066No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8450.20233075451413082No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC7830.18748518436043127No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT7600.18197795672276856No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7410.17742850780469935No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7350.17599183972530907No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7240.1733579482464269No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTGTTGATAATATCCTCTTTC6810.16306182701079655No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC6730.16114626957160955No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAACTATT6600.15803348873293058No Hit
ATTATCAACAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA6510.15587848661384518No Hit
TCCACAAAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTAGA6380.15276570577516624No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6370.15252626109526785No Hit
GTTTGGAAGTAATGATTAACTAGATGTTCAGGATAATAAGGTTTGATGCC6060.14510347601841808No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAATACAAAGATC5910.14151180581994238No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5710.1367229122219748No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT5700.1364834675420764No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5690.13624402286217802No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAA5590.13384957606319425No Hit
ATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTTGGA5490.13145512926421046No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTGTATTCTTCTACTGTACCTGTC5300.12690568034614125No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5210.1247506782270558No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5120.12259567610797038No Hit
CCTCAAGCTTACAGACCACCAAATACTCAAGAACAGTTTCTCTTCCAAAA4900.11732789315020604No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAAAGTATGTAAATAATG4800.11493344635122224No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC4740.11349677827183197No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTGTATTCTTCTACTG4580.1096656633934579No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4530.10846843999396599No Hit
GTGCTGGTGTTTGTGTGTATAATTGAATATTATTCAGCTTTTGTAAAGAA4430.1060739931949822No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA4420.10583454851508381No Hit
CTCCCAATACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG4220.10104565491711621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)