FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411403
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23370.5680561396003432No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT13690.33276373774620016No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC12210.29678927961147583No Hit
CCCTTACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG11210.27248221330422967No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10800.26251631611825876No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10590.25741183219373703No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9590.23310476588649084No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9530.2316463419080561No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8520.20709620493773745No Hit
ATTCTATGTTGGGGTGATTTAATGAATCTAGCCACCTGGGTGGGAGGTAA8360.20320707432857804No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7930.1927550358164622No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7810.18983818785959267No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7730.18789362255501296No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7240.17598316006446232No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7050.17136481746608556No Hit
GAGGCAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAA7030.17087867613994062No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6940.16869104017228848No Hit
CCTCCTGGATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6390.1553221537033031No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6210.15094688176799875No Hit
ATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCT6070.1475438924849843No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5980.14535625651733217No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGGACGACAGAAT5730.1392794899405206No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5720.13903641927744814No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5590.13587650065750614No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5590.13587650065750614No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC5410.13150122872220182No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5370.13052894606991197No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5320.12931359275454968No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG5280.12834131010225983No Hit
GGTTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5120.12445217949310045No Hit
TTTTTCGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT5110.12420910883002798No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5080.1234798968408106No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5020.12202147286237582No Hit
CCCGTAAAATGAGGCGCTATGTGTGGACTCTCTTTTATATAAGATCCCCG5010.12177840219930336No Hit
CCCCCAACTCCTCCCACTCGTTAAACACACAGTCTTTGAAGTATGCCTCA5000.12153533153623089No Hit
ATGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTA5000.12153533153623089No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4950.1203199782208686No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4950.1203199782208686No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4790.11643084761170919No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA4670.11351399965483966No Hit
CTCTAATTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTGC4600.11181250501333244No Hit
GATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCC4590.11156943435025996No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4510.10962486904568026No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT4500.10938179838260781No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4470.10865258639339043No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCGTG4350.10573573843652088No Hit
TCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4350.10573573843652088No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAACATGGGCCTAAAAATCAGA4320.1050065264473035No Hit
CCCGAAAAATGAGGCGCTATGTGTGGACTCTCTTTTATATAAGATCCCCG4320.1050065264473035No Hit
ATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4320.1050065264473035No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAAGCACCCGTG4300.10452038512115858No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4300.10452038512115858No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4270.10379117313194118No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA4230.10281889047965133No Hit
AGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4230.10281889047965133No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4230.10281889047965133No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT4190.1018466078273615No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4150.10087432517507165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)