FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411403
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23140.5624655143496766No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT14630.3556123800750116No Hit
CCCTTACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG10990.26713465871663555No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC10570.2569256908675921No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10180.2474459350077661No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9620.23383397787570823No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9310.22629878732046194No Hit
ATTCTATGTTGGGGTGATTTAATGAATCTAGCCACCTGGGTGGGAGGTAA8740.21244375952533162No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8670.2107422648838244No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8550.20782541692695483No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8380.203693215654723No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8240.2002902263717085No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7800.1895951171965202No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7540.1832752799566362No Hit
ATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCT6860.1667464748677088No Hit
CCTCCTGGATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6680.16237120293240448No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6640.16139892028011463No Hit
GTACAACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6360.1545929417140857No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6250.1519191644202886No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5680.13806413662515832No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5550.1349042180052163No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAAGCACCCGTG5530.13441807667907138No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5530.13441807667907138No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5500.13368886468985397No Hit
GAGGCAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAA5480.13320272336370909No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5270.12809823943918738No Hit
ATGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTA5240.12736902744996997No Hit
GGTTCAGATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5140.12493832081924537No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5090.12372296750388305No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4950.1203199782208686No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4860.11813234225321644No Hit
TTTTTCGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT4820.11716005960092658No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4820.11716005960092658No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4780.11618777694863675No Hit
CCCGTAAAATGAGGCGCTATGTGTGGACTCTCTTTTATATAAGATCCCCG4730.11497242363327444No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC4730.11497242363327444No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT4720.11472935297020197No Hit
CTCTAATTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTGC4720.11472935297020197No Hit
CCCCCAACTCCTCCCACTCGTTAAACACACAGTCTTTGAAGTATGCCTCA4700.11424321164405704No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACATAGCGCC4560.11084022236104259No Hit
ATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4550.1105971516979701No Hit
ATATGTTCTTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA4530.11011101037182519No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4520.10986793970875273No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4500.10938179838260781No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGGACGACAGAAT4480.10889565705646288No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCGTG4470.10865258639339043No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4430.10768030374110057No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4270.10379117313194118No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4260.10354810246886872No Hit
GTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAA4260.10354810246886872No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4250.10330503180579625No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4170.10136046650121658No Hit
GATACTGTTGACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCC4160.10111739583814411No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4160.10111739583814411No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAG4140.10063125451199918No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4130.10038818384892673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)