FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482638
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG32150.6661307232335623No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG20870.43241518487976494No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT19290.3996784339401373No Hit
CCCTTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT16010.33171859654647995No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG15960.3306826234154791No Hit
ATCCTAACCTCACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT15570.3226020329936723No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14310.29649551009245023No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13950.28903650354924393No Hit
AGTCTAGACTCCGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13410.2778479937344345No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTGAGGTT12290.25464219560001494No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11900.2465616051782081No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCACG10910.22604933718439077No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA10870.22522055867959007No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT10640.22045508227698604No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAG10610.21983349839838554No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10490.21734716288398345No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10480.21713996825778328No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10290.21320327035997994No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10030.2078162100787754No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA9770.20242914979757085No Hit
CTCCATGCCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGG9470.1962133110115656No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTG9410.19497014325436454No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9390.1945557540019642No Hit
GTTCTATCCTAACCTCACCAAATATTTGCCCTTAGATAAAGGCATCAAAC9260.19186222386136192No Hit
CTCCAAAAGACACCAAATACTCCAGAACAACTTCTCTTCCAAAAGTAAGA9170.18999747222556038No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC9150.18958308297316No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGCACCTGTC8910.1846104119443558No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8880.18398882806575528No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8740.18108810329895283No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8630.17880896241075092No Hit
GGAACATATTGTACAAAAAATCAGAGAATGTTTTCGGAAACTTCCCATAA8590.17798018390595022No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACT8440.1748722645129476No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA8150.16886362035314254No Hit
TCTCTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTATCCTTCTATTCG8120.16824203647454197No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA8070.16720606334354113No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT8070.16720606334354113No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8040.1665844794649406No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAAAG8010.1659628955863401No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7940.16451253320293885No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG7770.16099022455753587No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA7750.16057583530513553No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7570.15684633203353238No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7520.1558103589025315No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7500.15539596965013117No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6990.14482904371392222No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6840.1417211243209196No Hit
CTACTGCACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT6790.14068515118991873No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6790.14068515118991873No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC6760.1400635673113182No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACACACGGGCAAC6740.13964917805891788No Hit
GCTTTATTCTTCTACTGCACCTGTCTTTAATCCTGAATGGCAAACTCCCT6460.133847728525313No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6360.13177578226331121No Hit
TTCTTCTACTGCACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6290.13032541987991No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6250.1294966413751093No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTGAGGTTAG6230.12908225212270896No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA6210.1286678628703086No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC6200.12846066824410843No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6130.1270103058607072No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6030.12493835959870543No Hit
GGTCTATTTATGGGAAGTTTCCGAAAACATTCTCTGATTTTTTGTACAAT5990.12410958109390474No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAA5990.12410958109390474No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT5970.12369519184150439No Hit
CAATAGGTCTATTTATGGGAAGTTTCCGAAAACATTCTCTGATTTTTTGT5930.1228664133367037No Hit
CCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5930.1228664133367037No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG5840.12100166170090212No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5830.12079446707470194No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATGGGAAGTTTCCGAAAAC5820.12058727244850177No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5790.11996568856990125No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5760.11934410469130073No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGC5690.1178937423078995No Hit
ATTTATGGGAAGTTTCCGAAAACATTCTCTGATTTTTTGTACAATATGTT5620.11644337992449828No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5580.1156146014196976No Hit
ATTGACAACAGTACCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5550.11499301754109705No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC5530.11457862828869671No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5530.11457862828869671No Hit
GACGCAAATTGCACTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAG5500.11395704441009619No Hit
GGGTAACCACATTGTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAAT5460.11312826590529547No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5400.11188509814809443No Hit
GTCCAGAAGAGCCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5370.11126351426949391No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGCAAACCCTGT5310.11002034651229285No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACTC5230.10836278950269146No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5220.10815559487649129No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC5200.10774120562409092No Hit
ATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT5140.10649803786688988No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTAAGGCA5120.10608364861448953No Hit
CCTTATGAGTCCAAGGGATACTAACAGTTGGATTCCCGAGATTGAGATCT5090.10546206473588901No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAACTGTT5080.10525487010968884No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5080.10525487010968884No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5060.10484048085728848No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5040.10442609160488812No Hit
GTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA5020.10401170235248779No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4990.10339011847388727No Hit
TACCTTGATAGTCCAGAAGAGCCAACAAGAAGATGAGGCATAGCAGCAGG4970.10297572922148691No Hit
GGTTTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA4970.10297572922148691No Hit
GTCCAAGGGATACTAACAGTTGGATTCCCGAGATTGAGATCTTCTGCGAC4960.10276853459528675No Hit
GTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATG4950.10256133996908656No Hit
ATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCA4950.10256133996908656No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT4940.1023541453428864No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4910.10173256146428586No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4880.10111097758568534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)