FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482638
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG34820.7214516884290089No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG20770.4303432386177632No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT18860.3907690650135298No Hit
ATCCTAACCTCACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT14710.3047832951404572No Hit
CCCTTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT14410.298567456354452No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG14200.2942163692042483No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAGCACCCACG13970.28945089280164427No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13430.27826238298683487No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13130.2720465442008296No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTGAGGTT12990.26914581943402716No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG12040.24946232994501055No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10940.22667092106299133No Hit
AGTCTAGACTCCGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10920.22625653181059094No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTG10880.2254277533057903No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAG10540.21838313601498432No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA10540.21838313601498432No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT10280.21299607573377977No Hit
GTTCTATCCTAACCTCACCAAATATTTGCCCTTAGATAAAGGCATCAAAC10250.21237449185517923No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC9920.2055370691905735No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9750.2020147605451705No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9730.20160037129277014No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC9340.19351978087096333No Hit
CTCCAAAAGACACCAAATACTCCAGAACAACTTCTCTTCCAAAAGTAAGA9220.19103344535656122No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC9190.1904118614779607No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA9180.19020466685176055No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT8850.18336724418715475No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGCACCTGTC8740.18108810329895283No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8720.1806737140465525No Hit
TCTCTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTATCCTTCTATTCG7810.1618190030623366No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7720.159954251426535No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7640.1582966944169336No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACT7560.1566391374073322No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7540.15622474815493184No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA7540.15622474815493184No Hit
GTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTGATGCC7360.15249524488332872No Hit
GGAACATATTGTACAAAAAATCAGAGAATGTTTTCGGAAACTTCCCATAA7350.15228805025712852No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7340.15208085563092835No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7330.15187366100472818No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAAAG7280.1508376878737273No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7090.146900989975924No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG7060.14627940609732346No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA7040.14586501684492312No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7030.14565782221872295No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6970.14441465446152188No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA6870.14234270819952013No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6690.13861320492791698No Hit
CTCCATGCCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGG6590.13654125866591524No Hit
CTACTGCACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT6540.13550528553491437No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6390.13239736614191175No Hit
ATTTATGGGAAGTTTCCGAAAACATTCTCTGATTTTTTGTACAATATGTT6250.1294966413751093No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCATATTCATTTGAA6140.12721750048690736No Hit
TTCTTCTACTGCACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC6090.12618152735590651No Hit
GCTTTATTCTTCTACTGCACCTGTCTTTAATCCTGAATGGCAAACTCCCT6080.12597433272970632No Hit
GGTTTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA5970.12369519184150439No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5940.12307360796290387No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC5910.12245202408430335No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5850.1212088563271023No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA5810.1203800778223016No Hit
GGTCTATTTATGGGAAGTTTCCGAAAACATTCTCTGATTTTTTGTACAAT5780.11975849394370108No Hit
CAATAGGTCTATTTATGGGAAGTTTCCGAAAACATTCTCTGATTTTTTGT5750.11913691006510056No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5750.11913691006510056No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5690.1178937423078995No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5670.11747935305549916No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5640.11685776917689863No Hit
ATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTGAGGTTAG5600.11602899067209792No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACACACGGGCAAC5580.1156146014196976No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5560.11520021216729723No Hit
GGGTAACCACATTGTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAAT5500.11395704441009619No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5500.11395704441009619No Hit
GTCCAAGGGATACTAACAGTTGGATTCCCGAGATTGAGATCTTCTGCGAC5470.11333546053149567No Hit
GTTTCTCTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATG5470.11333546053149567No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5460.11312826590529547No Hit
CCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT5430.11250668202669495No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATGGGAAGTTTCCGAAAAC5410.11209229277429461No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5380.11147070889569408No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT5360.11105631964329374No Hit
ATTGACAACAGTACCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT5260.10898437338129198No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGC5240.10856998412889164No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5210.1079484002502911No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5200.10774120562409092No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT5200.10774120562409092No Hit
GATTAACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGC5170.1071196217454904No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGCAAACCCTGT5130.10629084324068971No Hit
CCTTTATCTAAGGGCAAATATTTGGTGAGGTTAGGATAGAACCTAGCAGG5030.10421889697868796No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACTC5030.10421889697868796No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTAAGGCA4980.10318292384768708No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4950.10256133996908656No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA4910.10173256146428586No Hit
GAATATGGTGACCCACAAAATGAGGCGCTGCGAGTAGTTTCTCTCTTATA4850.10048939370708482No Hit
ATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC4840.10028219908088463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)