FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653034
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN47100.7212488170600612No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG29360.4495937424391379No Hit
ATCCTAAACGTACCAAATATTTGCCATTAGATAAAGGCATCAAACCTTAT26300.40273553903778364No Hit
CTCTTACAGTTAACGAAAAGAGAAGATTAAAATTGATTATGCCTGCCAGG15930.24393829417763851No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13680.20948373285311334No Hit
GTTTAGGATAGAACCTGGCAGGCATAATCAATTTTAATCTTCTCTTTTCG13050.19983645568224626No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA12150.18605463115243617No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCTTCTTTTCCTCAC11660.1785511933528729No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGTCTAAAAATCAGACAACTATT11540.1767136167488982No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCTTCTTTTC10780.16507563159039193No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT10020.15343764643188562No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10010.15328451504822108No Hit
CTCCAAAAGACACCAAATACTCAAGAACAACGTGTCTTCCAAAAGTTAGA9650.14777178523629705No Hit
GGATAATAAGGTTTGATGCCTTTATCTAATGGCAAATATTTGGTACGTTT9570.14654673416698058No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9500.14547481448132868No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAGAGAAGATTAAAATTGATTA9180.14057461020406287No Hit
GAACAATATGTAGGCCCTCTTACAGTTAACGAAAAGAGAAGATTAAAATT8770.13429622347381606No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCA8700.13322430378816416No Hit
GTTCTATCCTAAACGTACCAAATATTTGCCATTAGATAAAGGCATCAAAC8580.1313867271841895No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAGAGAAGATTAAAATTGATTATG8580.1313867271841895No Hit
GTCAATAGATGTGAACAATATGTAGGCCCTCTTACAGTTAACGAAAAGAG8420.12893662504555659No Hit
CTTTAATCCTGACTGGCAAACTCCTTCTTTTCCTCACATTCATTTGAAAG8250.12633339152325912No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATCCGTTGGCATACT8000.12250510693164521No Hit
ATTGACAACAGTGCCAACAGCGCCTCCTCCTGCCGCCACCAATCGGCAGT7900.12097379309499966No Hit
CAATAGATGTGAACAATATGTAGGCCCTCTTACAGTTAACGAAAAGAGAA7820.11974874202568321No Hit
CTCAAGAACAACGTGTCTTCCAAAAGTTAGACAAGAAATGTGAAACCACA7530.11530793189941106No Hit
AGATAAAGGCATCAAACCTTATTATCCTGAACATACAGTTAATCATTACT7430.11377661806276548No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7410.11347035529543639No Hit
CCTTTATCTAATGGCAAATATTTGGTACGTTTAGGATAGAACCTGGCAGG7220.1105608590058098No Hit
CTGTAAGAGGGCCTACATATTGTTCACATCTATTGACAATATCCTCTTTC6910.10581378611220855No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT6870.10520126057755033No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCTT6730.10305742120624653No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6700.10259802705525287No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6550.10030105630028452No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)