FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653034
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46720.715429824480808No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG31640.4845076979146568No Hit
ATCCTAAACGTACCAAATATTTGCCATTAGATAAAGGCATCAAACCTTAT22000.3368890440620243No Hit
GTTTAGGATAGAACCTGGCAGGCATAATCAATTTTAATCTTCTCTTTTCG13210.20228655782087915No Hit
CTCTTACAGTTAACGAAAAGAGAAGATTAAAATTGATTATGCCTGCCAGG12820.19631443385796143No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA11040.1690570475656704No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10640.16293179221908813No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGTCTAAAAATCAGACAACTATT10520.16109421561511345No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCTTCTTTTC9560.14639360278331603No Hit
CTCCAAAAGACACCAAATACTCAAGAACAACGTGTCTTCCAAAAGTTAGA9370.14348410649368945No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9040.1384307708327591No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAGAGAAGATTAAAATTGATTA8930.13674632561244895No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCA8650.13245864686984138No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCTTCTTTTCCTCAC8560.13108046441686036No Hit
GGATAATAAGGTTTGATGCCTTTATCTAATGGCAAATATTTGGTACGTTT8240.12618026013959457No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7980.1221988441643161No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT7850.12020813617667687No Hit
GTTCTATCCTAAACGTACCAAATATTTGCCATTAGATAAAGGCATCAAAC7800.11944247925835408No Hit
GAACAATATGTAGGCCCTCTTACAGTTAACGAAAAGAGAAGATTAAAATT7440.11392974944643006No Hit
CTGTAAGAGGGCCTACATATTGTTCACATCTATTGACAATATCCTCTTTC7320.11209217284245537No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATCCGTTGGCATACT7270.11132651592413259No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAGAGAAGATTAAAATTGATTATG7260.11117338454046802No Hit
GTTTGGAAGTAATGATTAACTGTATGTTCAGGATAATAAGGTTTGATGCC6910.10581378611220855No Hit
CCTTTATCTAATGGCAAATATTTGGTACGTTTAGGATAGAACCTGGCAGG6870.10520126057755033No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAATGGCAAATATTTGGTA6790.10397620950823387No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)