FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289561
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT17080.589858440881196No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA16550.5715548709943673No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTAACATT10960.37850401124460825No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10630.36710744886224317No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10360.3577829887312173No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10220.3529480834780927No Hit
GTACAGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG9680.3342991632160408No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCCAGG9650.3332631120903713No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8650.298728074568053No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7950.27455354830243023No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7430.25659532879082475No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7280.251415073162477No Hit
ATATGTTCCTGTGGCAATGAGCCCCAACACCCAATTACATATCCCATGAA6950.24001851078011197No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGGACACCCGTG6910.23863710927921922No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6870.2372557077783265No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA6410.2213695905180601No Hit
ATGTATACTTGCGGGAGAGCACAACAGAATTGTCAGTCCCGATGAGTTTC6240.215498634139266No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6200.2141172326383733No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6160.21273583113748054No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5930.20479277250734731No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTCACGGGTCACCATATT5840.2016846191303387No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5770.1992671665037764No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTGAATGGCAAACTCCTT5700.19684971387721414No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5540.1913241078736432No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5520.19063340712319685No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC5420.18717990337096502No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5370.1854531514948491No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG5150.17785544323993907No Hit
ATATGCAGGAGGAGTGCGCATCCACACTCCAAAAGACACCAAATATTCAA5140.1775100928647159No Hit
GCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGAAA5110.17647404173904635No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4960.17129378611069862No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4950.17094843573547544No Hit
GATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGAGACTA4930.17025773498502908No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACAACAGAAT4820.16645888085757404No Hit
AAAGAGGACATTGCTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGT4820.16645888085757404No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4770.16473212898145814No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC4740.1636960778557886No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4680.1616239756044495No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4620.15955187335311038No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4620.15955187335311038No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4610.1592065229778872No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4540.15678907035132494No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4530.15644371997610176No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4430.15299021622386993No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAGCAATGTCCTCTTTC4430.15299021622386993No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4410.15229951547342357No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTA4410.15229951547342357No Hit
CTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4410.15229951547342357No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACAT4250.14677390946985264No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4200.14504715759373674No Hit
CTACGGTACCTTGCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTGAC4200.14504715759373674No Hit
GTAGTCAACTATGTCAACGTTAATATGGGCATAAAAATCAGACAACTATT4190.14470180721851356No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4130.14262970496717445No Hit
ATGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTC4040.1395215515901658No Hit
ATTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGGAAAGT4000.13814015008927308No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3960.13675874858838033No Hit
ATGTAATTGGGTGTTGGGGCTCATTGCCACAGGAACATATTGTACAAAAA3920.1353773470874876No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3910.13503199671226443No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3880.1339959455865949No Hit
TCCCTGTACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3870.1336505952113717No Hit
GTCCCGTGCTGGTTGTGGATGATCCTGGAATTAGAGGACAAACGGGCAAC3820.13192384333525578No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACGTT3800.13123314258480942No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC3800.13123314258480942No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA3770.13019709145913988No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG3760.12985174108391667No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC3730.12881568995824713No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3730.12881568995824713No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA3660.12639823733168487No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTA3650.1260528869564617No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTCATGACTTCTTTCCTTCTATTCG3650.1260528869564617No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3610.12467148545556896No Hit
CCCCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGC3600.12432613508034578No Hit
ATCCCAATGTTACCAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT3600.12432613508034578No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3560.12294473357945303No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3540.12225403282900667No Hit
GCTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAG3530.12190868245378347No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCCAGGT3500.12087263132811393No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCCGTG3450.11914587945199802No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3450.11914587945199802No Hit
GTTTTATCCCAATGTTACCAAATATTTGCCCTTAGATAAAGGGATCAAAC3440.11880052907677484No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG3400.11741912757588212No Hit
CATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGCTT3390.11707377720065894No Hit
ACGTTAATATGGGCATAAAAATCAGACAACTATTGTGGTTTCACATTTCC3380.11672842682543576No Hit
CCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGAAAC3360.11603772607498938No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3320.11465632457409666No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCACTCAGGCA3310.11431097419887346No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3290.1136202734484271No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3280.11327492307320391No Hit
ACCTTGCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTGACATTCATT3280.11327492307320391No Hit
ATCCTGGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGA3270.11292957269798073No Hit
GGGTAAAGGTTCAGATACTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA3220.11120282082186482No Hit
GATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGC3210.11085747044664164No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT3180.10982141932097209No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA3180.10982141932097209No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG3180.10982141932097209No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA3140.10844001782007937No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3110.10740396669440982No Hit
CCACAATAGTTGTCTGATTTTTATGCCCATATTAACGTTGACATAGTTGA3080.10636791556874027No Hit
GCTCTGTACAGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA3070.10602256519351709No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA3060.10567721481829391No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGG3060.10567721481829391No Hit
TTGCTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAAC3060.10567721481829391No Hit
ATCTAAGGGCAAATATTTGGTAACATTGGGATAAAACCTGGCAGGCATAA3030.10464116369262434No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3020.10429581331740116No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3010.10395046294217798No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC2970.10256906144128525No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCCAGGTTTTAT2960.10222371106606207No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA2950.10187836069083889No Hit
TTATTCTTCTACGGTACCTTGCTTTAATCCTGAATGGCAAACTCCTTCTT2940.10153301031561571No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA2940.10153301031561571No Hit
CTTCTACGGTACCTTGCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCT2910.10049695918994617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)