FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289561
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT16680.5760444258722688No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA15820.546344293603075No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTAACATT11920.4116576472660337No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9830.3394794188443886No Hit
GTACAGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG9390.32428400233456856No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9080.3135781407026499No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8700.30045482644416893No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCCAGG8660.2990734249432762No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGGACACCCGTG8290.28629546106001846No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8240.28456870918390254No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7850.2711000445501984No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7390.25521392728993203No Hit
ATATGTTCCTGTGGCAATGAGCCCCAACACCCAATTACATATCCCATGAA6860.23691035740310332No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6530.2255137950207383No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT6500.22447774389506872No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC6180.2134265318879269No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6120.21135442963658782No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5930.20479277250734731No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC5590.1930508597497591No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5590.1930508597497591No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTGAATGGCAAACTCCTT5550.1916694582488664No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5520.19063340712319685No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5380.18579850187007227No Hit
ATATGCAGGAGGAGTGCGCATCCACACTCCAAAAGACACCAAATATTCAA5140.1775100928647159No Hit
GCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGAAA5140.1775100928647159No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTCACGGGTCACCATATT4970.1716391364859218No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4930.17025773498502908No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4860.16784028235846676No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4790.1654228297319045No Hit
ATGTATACTTGCGGGAGAGCACAACAGAATTGTCAGTCCCGATGAGTTTC4740.1636960778557886No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG4680.1616239756044495No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC4660.16093327485400313No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC4590.15851582222744084No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAGCAATGTCCTCTTTC4580.15817047185221766No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4530.15644371997610176No Hit
GATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGAGACTA4500.1554076688504322No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4460.15402626734953948No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4400.1519541650982004No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4360.15057276359730765No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4340.14988206284686129No Hit
CTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT4290.14815531097074536No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4260.14711925984507582No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4250.14677390946985264No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4140.14297505534239763No Hit
GTTTTATCCCAATGTTACCAAATATTTGCCCTTAGATAAAGGGATCAAAC4120.14228435459195127No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACGTT4090.1412483034662817No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACAT4050.13986690196538898No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4000.13814015008927308No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTA3990.1377947997140499No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACAACAGAAT3980.13744944933882672No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTGGTA3970.13710409896360354No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3890.13434129596181807No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC3830.13226919371047896No Hit
GTCCCGTGCTGGTTGTGGATGATCCTGGAATTAGAGGACAAACGGGCAAC3790.13088779220958624No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA3770.13019709145913988No Hit
ATGTAATTGGGTGTTGGGGCTCATTGCCACAGGAACATATTGTACAAAAA3730.12881568995824713No Hit
CCCCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGC3650.1260528869564617No Hit
GCTCTGTACAGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA3560.12294473357945303No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3550.12259938320422985No Hit
CTACGGTACCTTGCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTGAC3520.1215633320785603No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3440.11880052907677484No Hit
GATTAACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGC3430.11845517870155166No Hit
ATTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGGAAAGT3430.11845517870155166No Hit
ATGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTC3420.11810982832632848No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3390.11707377720065894No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3370.11638307645021256No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCACTCAGGCA3370.11638307645021256No Hit
TCCCTGTACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3350.1156923756997662No Hit
AAAGAGGACATTGCTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGT3350.1156923756997662No Hit
ATCCCAATGTTACCAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT3350.1156923756997662No Hit
GTAGTCAACTATGTCAACGTTAATATGGGCATAAAAATCAGACAACTATT3320.11465632457409666No Hit
CGTGTGGACTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAA3310.11431097419887346No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTCATGACTTCTTTCCTTCTATTCG3290.1136202734484271No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG3290.1136202734484271No Hit
CTTTATCTAAGGGCAAATATTTGGTAACATTGGGATAAAACCTGGCAGGC3200.11051212007141846No Hit
GGGTAAAGGTTCAGATACTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA3200.11051212007141846No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCCAGGT3140.10844001782007937No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC3120.107749317069633No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT3110.10740396669440982No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3110.10740396669440982No Hit
CTTTTGGAGTGTGGATGCGCACTCCTCCTGCATATAGACCACAAAATGCC3100.10705861631918664No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA3080.10636791556874027No Hit
CCCTTAACTTCATGGGATATGTAATTGGGTGTTGGGGCTCATTGCCACAG3080.10636791556874027No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3060.10567721481829391No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3020.10429581331740116No Hit
GTTCAGATACTGTTTACATAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3000.1036051125669548No Hit
CTTCTACGGTACCTTGCTTTAATCCTGAATGGCAAACTCCTTCTTTTCCT2980.10291441181650843No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTACGCGGGACGTCCTTTGTTTA2970.10256906144128525No Hit
GCTGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAG2950.10187836069083889No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA2930.10118765994039253No Hit
GTCTATATGCAGGAGGAGTGCGCATCCACACTCCAAAAGACACCAAATAT2930.10118765994039253No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA2930.10118765994039253No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT2930.10118765994039253No Hit
CTGTAGATCTTGTTCCCAAGAATATGGTGACCCGTGAAATGAGGCGCTAC2910.10049695918994617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)