FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314975
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCATACCAAATATTTACCTTTAGATAAAGGCATTAAACCTTAT18020.5721088975315501No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG17020.540360346059211No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA13920.4419398364949599No Hit
CCCTTTACACAATGTGGTTACCCAGCCTTAATGCCTTTGTATGCATGTAT12080.38352250178585606No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG11210.355901262004921No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG10660.33843955869513453No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10490.3330423049448369No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC10440.3314548773712199No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9940.3155806016350504No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT9600.3047860941344551No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG8950.28414953567743473No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC8630.2739899992062862No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGA8090.2568457814112231No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7860.24954361457258512No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7750.2460512739106278No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7730.24541630288118108No Hit
GTTCTATCCTAACCATACCAAATATTTACCTTTAGATAAAGGCATTAAAC7660.24319390427811732No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT7440.23620922295420269No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7420.23557425192475595No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7300.23176442574807526No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAAGGTAAATATTTGGTATGGTT6990.22192237479165014No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6960.22096991824747994No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG6880.21843003412969283No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT6860.21779506310024604No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6540.20763552662909757No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6530.20731804111437413No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6480.2057306135407572No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA6440.20446067148186367No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6190.19652353361377886No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG6190.19652353361377886No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5970.18953885228986428No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5860.18604651162790697No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5750.18255417096594967No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5690.18064925787760933No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG5650.17937931581871577No Hit
ATCTAAAGGTAAATATTTGGTATGGTTAGGATAGAACCTAGCAGGCATAA5640.17906183030399236No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAA5610.17810937375982222No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG5480.17398206206841813No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA5310.16858480831812048No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT5300.1682673228033971No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5240.16636240971505675No Hit
GGTAAATATTTGGTATGGTTAGGATAGAACCTAGCAGGCATAATCAATTT5080.16128264147948249No Hit
GTATACATGCATACAAAGGCATTAAGGCTGGGTAACCACATTGTGTAAAG4880.15493293118501467No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4860.1542979601555679No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4840.1536629891261211No Hit
ATGTAAGACCTTGGGCAAGACCTGTTGGGCGTTCACGGTGGTCTCCATGC4800.15239304706722756No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA4710.14953567743471705No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGTAAATATTTGGTA4700.14921819191999364No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG4670.1482657353758235No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG4490.14255099611080244No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4430.1406460830224621No Hit
GGTCTATTTATAGGAATTTTTCGAAAACATTGTTTGATTTTGTGTACAAT4390.13937614096356854No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4370.13874116993412175No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG4290.13620128581633462No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA4280.13588380030161124No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4270.13556631478688783No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT4130.13112151758076038No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4130.13112151758076038No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC4100.1301690610365902No Hit
ATTTATAGGAATTTTTCGAAAACATTGTTTGATTTTGTGTACAATATGTT4070.12921660449242003No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGT4040.12826414794824986No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT3970.1260417493451861No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3930.12477180728629256No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA3910.12413683625684578No Hit
CAATAGGTCTATTTATAGGAATTTTTCGAAAACATTGTTTGATTTTGTGT3910.12413683625684578No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3900.12381935074212239No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT3840.12191443765378204No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC3820.12127946662433527No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3820.12127946662433527No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA3820.12127946662433527No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3800.12064449559488848No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3800.12064449559488848No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC3770.11969203905071832No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT3760.11937455353599492No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTAAGGCA3750.11905706802127153No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3700.11746964044765458No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3620.11492975632986745No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAAGAGTCCTCTTATGT3600.11429478530042066No Hit
CCTTTAGATAAAGGCATTAAACCTTATTATCCTGAACACATAGTTAATCA3580.1136598142709739No Hit
ATATTTACCTTTAGATAAAGGCATTAAACCTTATTATCCTGAACACATAG3520.11175490118263355No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT3520.11175490118263355No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3520.11175490118263355No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC3480.11048495912373997No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3420.10858004603539963No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC3420.10858004603539963No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT3410.10826256052067623No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT3370.10699261846178268No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3320.10540519088816573No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA3320.10540519088816573No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT3310.10508770537344234No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG3280.10413524882927218No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG3250.10318279228510199No Hit
ATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTA3250.10318279228510199No Hit
ACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACAT3250.10318279228510199No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3240.1028653067703786No Hit
CAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATT3230.1025478212556552No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT3220.10223033574093184No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3220.10223033574093184No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTTTGCCAAGTGTTTGCTG3210.10191285022620844No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG3190.10127787919676165No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTCC3180.10096039368203825No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3160.10032542265259149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)