FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314975
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG22260.7067227557742678No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA12210.3876498134772601No Hit
ATCCTAACCATACCAAATATTTACCTTTAGATAAAGGCATTAAACCTTAT12160.38606238590364317No Hit
CCCTTTACACAATGTGGTTACCCAGCCTTAATGCCTTTGTATGCATGTAT9760.30986586237002933No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG9540.3028811810461148No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC9130.28986427494245576No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG8720.27684736883879674No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8600.27303754266211605No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT8070.25621081038177634No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7900.2508135566314787No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG7490.23779665052781965No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7410.23525676641003254No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7360.23366933883641558No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7290.23144694023335186No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7180.22795459957139458No Hit
GTTCTATCCTAACCATACCAAATATTTACCTTTAGATAAAGGCATTAAAC6930.2200174617033098No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT6770.21493769346773553No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAAGGTAAATATTTGGTATGGTT6650.21112786729105484No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6610.2098579252321613No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6590.2092229542027145No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGA6490.2060480990554806No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG6380.2025557583935233No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6300.2000158742757362No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6140.19493610604016193No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG6010.19080879434875783No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5650.17937931581871577No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGTAAATATTTGGTA5620.1784268592745456No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5580.17715691721565202No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5400.171442177950631No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5400.171442177950631No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA5380.1708072069211842No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5370.17048972140646085No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5130.16287006905309945No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5010.15906024287641876No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5000.15874275736169538No Hit
GTATACATGCATACAAAGGCATTAAGGCTGGGTAACCACATTGTGTAAAG4960.1574728153028018No Hit
ATCTAAAGGTAAATATTTGGTATGGTTAGGATAGAACCTAGCAGGCATAA4920.15620287324390825No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG4530.14382093816969602No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4460.14159853956663226No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG4440.1409635685371855No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4310.1368362568457814No Hit
GGTAAATATTTGGTATGGTTAGGATAGAACCTAGCAGGCATAATCAATTT4270.13556631478688783No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT4270.13556631478688783No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC4230.13429637272799427No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAA4210.13366140169854748No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA4180.13270894515437734No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA4110.1304865465513136No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG4090.1298515755218668No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4080.12953409000714344No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4010.1273116914040797No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3950.12540677831573935No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG3930.12477180728629256No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG3930.12477180728629256No Hit
GGTCTATTTATAGGAATTTTTCGAAAACATTGTTTGATTTTGTGTACAAT3780.1200095245654417No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3750.11905706802127153No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3720.11810461147710134No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG3720.11810461147710134No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3700.11746964044765458No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGT3680.11683466941820779No Hit
ATTTATAGGAATTTTTCGAAAACATTGTTTGATTTTGTGTACAATATGTT3670.1165171839034844No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT3660.116199698388761No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT3600.11429478530042066No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC3600.11429478530042066No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT3570.1133423287562505No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3470.11016747360901658No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG3450.10953250257956981No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3450.10953250257956981No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC3390.10762758949122947No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA3360.10667513294705928No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTAAGGCA3340.10604016191761252No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3270.10381776331454878No Hit
ATGTAAGACCTTGGGCAAGACCTGTTGGGCGTTCACGGTGGTCTCCATGC3260.10350027779982539No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3260.10350027779982539No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA3260.10350027779982539No Hit
GCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT3190.10127787919676165No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT3180.10096039368203825No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3180.10096039368203825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)