FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413228
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23850.5771632125606203No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGCATGTAGTTAATCATTAC13850.33516605844715264No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12150.2940265422478632No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12050.29160657070672846No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10670.25821096343906996No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9980.24151315980524066No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC9280.22457335901729797No Hit
CTTTCTCTAAGGGGAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC8280.20037364360595117No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8240.19940565498949733No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT6790.16431606764304452No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6700.1621380932560233No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6650.16092810748545597No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC6530.15802414163609435No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT6410.15512017578673276No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6260.15149021847503075No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5850.14156833515637854No Hit
GGATAATACGGTTTGATCCCTTTCTCTAAGGGGAAATATTTAGTAACATT5570.13479241484120147No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCGTC5560.13455041768708798No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5520.13358242907063414No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG5400.13067846322127252No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5360.12971047460481863No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5340.1292264802965917No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5330.12898448314247823No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCGAAAACATATTTTGATTTTTTCT5160.1248705315225493No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTATCTCTTCCAAAAGTAAGA5040.12196656567318767No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT4990.12075657990262033No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4910.11882060266971262No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4890.11833660836148567No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4800.11615863397446445No Hit
CCTTCTTTTCCTGACATTCATTTGCAGGAGGACATTGTTGATAGATGTAA4640.11228667950864898No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4620.11180268520042205No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4600.11131869089219511No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4600.11131869089219511No Hit
GTTTACAGGAAGTTTCCGAAAACATATTTTGATTTTTTCTACAATATGTT4520.10938271365928737No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4400.10647874780992576No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTTTG4350.10526876203935842No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG4290.10381677911467761No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA4290.10381677911467761No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4280.10357478196056415No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAAAAGGAGTTT4160.10067081611120253No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGCATTGTGGGTCT4150.10042881895708905No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4150.10042881895708905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)