FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403057
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18490.4587440486085095No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATCAAACCTTAT15420.3825761616843275No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14610.3624797485219212No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14600.36223164465571867No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCCAGG13560.33642884257065375No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC12020.29822084717546155No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10100.2505849048645725No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9700.24066075021647063No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG9130.22651882984292543No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA9060.22478210277950764No Hit
GGTTAGGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8810.21857950612444396No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAA8670.2151060519976083No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8470.21014397467355733No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8280.20543000121570892No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT7850.19476153496899942No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7730.19178428857456886No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC7720.1915361847083663No Hit
CATCTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7650.1897994576449485No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTACTGACTTCTTTCCTTCCATTCG7410.18384496485608734No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7390.18334875712368226No Hit
GATACTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC7170.17789047206722622No Hit
CTATAAGGGGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATACTC7100.1761537450038084No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCG6690.16598148648950395No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6390.15853837050342756No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6360.15779405890481993No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6200.15382439704557915No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACGTTACCGCAG6100.1513433583835537No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT6030.14960663132013585No Hit
GCATCAAACCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT5880.14588507332709766No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5720.1419154114678569No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5670.14067489213684417No Hit
ATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGC5530.1372014380100085No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATCAAAC5460.13546471094659068No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5420.1344722954817805No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5360.1329836722845652No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCTGTC5230.12975832202393212No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5190.1287659065591219No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5160.12802159496051427No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC5130.12727728336190663No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA5040.1250443485660837No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAGTTGATGA5030.12479624469988115No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5010.12430003696747606No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4940.12256330990405824No Hit
GGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4900.12157089443924805No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4890.12132279057304549No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG4860.12057847897443785No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4760.1180974403124124No Hit
CTTTTACACAATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTATA4680.11611260938279203No Hit
CCCTTATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGAAACTG4630.11487209005177927No Hit
GTTGTCAATAGGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4530.11239105138975382No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG4500.11164673979114617No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4500.11164673979114617No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4430.10991001272772834No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4430.10991001272772834No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATCATT4340.10767707793190541No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4340.10767707793190541No Hit
GGATAATAAGGTTTGATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT4290.10643655860089268No Hit
AACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGC4270.10594035086848759No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4190.1039555199388672No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4170.10345931220646211No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4090.10147448127684174No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAAATCATTAACCTAGTCTCCTCC4040.10023396194582901No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)