FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403057
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG17830.4423691934391414No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14510.35999870985989574No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12420.3081450018235634No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC11740.29127393892179027No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATCAAACCTTAT10900.27043321416077626No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCCAGG10550.26174957884368716No Hit
GGTTAGGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9120.2262707259767229No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8880.22031623318786178No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8660.21485794813140574No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA8100.2009641316240631No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7770.19277670403937905No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG7450.18483738032089753No Hit
CTATAAGGGGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATACTC7230.1793790952644415No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7000.17367270634178295No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6960.17268029087697273No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAA6930.1719359792783651No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCGTTGACATACT6430.15953078596823775No Hit
CATCTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6340.15729785117241482No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6290.15605733184140208No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5830.14464455399608492No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACGTTACCGCAG5820.14439645012988236No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5730.14216351533405944No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT5700.1414192037354518No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5590.1386900612072238No Hit
GATACTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCC5330.13223936068595757No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5230.12975832202393212No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5220.12951021815772956No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5190.1287659065591219No Hit
CTTTTACACAATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTATA5140.1275253872281092No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCG4990.12380382923507097No Hit
ATGTGGATATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGC4900.12157089443924805No Hit
GGATAGAACCTGGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4870.1208265828406404No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG4710.11685692098139966No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4710.11685692098139966No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4700.1166088171151971No Hit
GCATCAAACCTTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACT4680.11611260938279203No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT4660.11561640165038692No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4590.1138796745869691No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATCAAAC4450.11040622046013342No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTACTGACTTCTTTCCTTCCATTCG4450.11040622046013342No Hit
GTACAACATCGTGAGGCCCTTTTTACCTCTATTACCAATTTTCTTCTGTC4430.10991001272772834No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4420.1096619088615258No Hit
GGATAATAAGGTTTGATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT4390.10891759726291816No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4380.1086694933967156No Hit
GTCTACGTCCCCTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4280.10618845473469013No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG4280.10618845473469013No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA4280.10618845473469013No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4230.10494793540367739No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGTACCTGTC4200.10420362380506976No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4080.10122637741063918No Hit
GTGTAAACAGTATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4070.10097827354443666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)