FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441819
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT27280.6174474162496407No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT20210.4574271364518049No Hit
CCTTTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA17610.3985795088033787No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14710.3329417702724419No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA14690.33248909621360784No Hit
CCTGGTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA14520.32864136671351846No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13320.3014809231834756No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11650.26368263927083263No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10800.24444399177038562No Hit
TGATCAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG10800.24444399177038562No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10490.23742754385845788No Hit
TCTAAGGTACCTTGCTTTAATCCCAAATGGCAAACTCCTTCTTTTCCTGA9950.22520534426993857No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9200.20823006706366184No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9090.20574035974007454No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9050.20483501162240647No Hit
TCTACGGTACCTTGCTTTAATCCCAAATGGCAAACTCCTTCTTTTCCTGA8580.19419717123980634No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8100.1833329938277892No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA8050.18220130868070408No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8000.18106962353361897No Hit
CTCCAAAAGATACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA7980.18061694947478493No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7800.1765428829452785No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7330.16590504256267838No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTTTCGCCAACTTACAAGGCC7030.15911493168016766No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6940.15707789841541445No Hit
CTTCTACGGTACCTTGCTTTAATCCCAAATGGCAAACTCCTTCTTTTCCT6860.15526720218007828No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6790.1536828429741591No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6630.1500614505034867No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6480.14666639506223136No Hit
CAGACACATCCAGCGATAACCAGGACAAATTGGAGGACAACAGGTTGGTG6420.14530837288572923No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6340.14349767665039304No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT6180.13987628417972067No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTGTTATGGTTTCACATTTCCT6160.1394236101208866No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6090.13783925091496743No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6060.13716023982671638No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5890.13331251032662697No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5880.13308617329720995No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5740.1299174548853716No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATCCTTGATCTTC5740.1299174548853716No Hit
TGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATG5710.12923844379712054No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5630.12742774756178435No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC5460.12358001806169495No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5390.12199565885577578No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5270.1192796145027715No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5080.11497921094384804No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTCACGGTGGTCTC5050.11430019985559697No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5050.11430019985559697No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAAT5030.11384752579676294No Hit
GTCAGAGCCCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAG4840.10954712223783948No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA4740.10728375194366924No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4740.10728375194366924No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4700.10637840382600114No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4680.1059257297671671No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATATTCAA4660.10547305570833304No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4540.10275701135532876No Hit
GCTATGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACATCACCA4540.10275701135532876No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4540.10275701135532876No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4510.1020780002670777No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4430.10026730403174151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)