FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441819
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT21240.4807398504817584No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT16530.3741351096263402No Hit
CCTTTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA13840.3132504487131608No Hit
CCTGGTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA10910.24693369909397286No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10770.24376498068213454No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10760.2435386436527175No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10720.24263329553504942No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10100.22860039971119395No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9730.22022592962276408No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9160.20732471894599372No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8730.19759222668106172No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8720.19736588965164467No Hit
TGATCAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG8620.19510251935747444No Hit
TCTAAGGTACCTTGCTTTAATCCCAAATGGCAAACTCCTTCTTTTCCTGA7520.17020544612160182No Hit
CTCCAAAAGATACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA7230.16364167226850815No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7110.16092562791550385No Hit
TCTACGGTACCTTGCTTTAATCCCAAATGGCAAACTCCTTCTTTTCCTGA6290.14236599150330792No Hit
CTTCTACGGTACCTTGCTTTAATCCCAAATGGCAAACTCCTTCTTTTCCT6080.13761291388555041No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6070.1373865768561334No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5780.1308228030030397No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5620.12720141053236733No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5500.12448536617936305No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5380.12176932182635875No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5300.11995862559102256No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT5250.11882694044393743No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5180.11724258123801828No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5100.11543188500268209No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATCCTTGATCTTC5100.11543188500268209No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4830.10932078520842245No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4750.10751008897308625No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4730.10705741491425222No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA4650.10524671867891602No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4650.10524671867891602No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4620.10456770759066496No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4600.10411503353183092No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTTTCGCCAACTTACAAGGCC4530.10253067432591173No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATATTCAA4510.1020780002670777No Hit
TGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATG4450.10071997809057555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)